Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CEBP Alpha/CEBPA antibody [EP708Y] (ab40761)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP708Y] to CEBP Alpha/CEBPA
  • Suitable for: WB, ICC/IF, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-CEBP Alpha/CEBPA antibody [EP708Y]
    See all CEBP Alpha/CEBPA primary antibodies

  • Description

    Rabbit monoclonal [EP708Y] to CEBP Alpha/CEBPA

  • Host species

    Rabbit

  • Specificity

    This antibody recognizes isoform1 and isoform 4.

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human CEBP Alpha/CEBPA aa 1-100 (N terminal). The exact sequence is proprietary.

  • Positive control

    • U937+LPS cell lysate, HeLa cells.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product was previously labelled as CEBP Alpha.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-CEBP Alpha/CEBPA antibody [EP708Y] (ab40761)
    Western blot - Anti-CEBP Alpha/CEBPA antibody [EP708Y] (ab40761)
    Anti-CEBP Alpha/CEBPA antibody [EP708Y] (ab40761) at 1/1000 dilution + U937 + LPS cell lysate at 10 µg

    Predicted band size: 43 kDa
    Observed band size: 30,43 kDa
    why is the actual band size different from the predicted?



    We are unsure as to the identity of the 30kda band.

  • Immunocytochemistry/ Immunofluorescence - Anti-CEBP Alpha/CEBPA antibody [EP708Y] (ab40761)
    Immunocytochemistry/ Immunofluorescence - Anti-CEBP Alpha/CEBPA antibody [EP708Y] (ab40761)
    ab40761 (1/250) staining HeLa cells using immunofluorescence.
  • Flow Cytometry - Anti-CEBP Alpha/CEBPA antibody [EP708Y] (ab40761)
    Flow Cytometry - Anti-CEBP Alpha/CEBPA antibody [EP708Y] (ab40761)

    Overlay histogram showing HeLa cells stained with ab40761 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab40761, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • Western blot - Anti-CEBP Alpha/CEBPA antibody [EP708Y] (ab40761)
    Western blot - Anti-CEBP Alpha/CEBPA antibody [EP708Y] (ab40761) This image is courtesy of an anonymous Abreview
    Anti-CEBP Alpha/CEBPA antibody [EP708Y] (ab40761) at 1/5000 dilution + Human MCF-7 whole cell lysate at 100000 cells

    Secondary
    HRP-conjugated Donkey anti-rabbit IgG polyclonal at 1/2000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 43 kDa
    Observed band size: 40 kDa why is the actual band size different from the predicted?


    Exposure time: 1 minute


    Blocked with 5% Milk for 1 hour at 24°C.

    We are unsure as to the identity of the 30kda band.

    See Abreview

  • Anti-CEBP Alpha/CEBPA antibody [EP708Y] (ab40761)
    Anti-CEBP Alpha/CEBPA antibody [EP708Y] (ab40761)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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