Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸

Order now and get it on Tuesday March 02, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR17059] to ATM
Suitable for: IP, WB
Reacts with: Mouse, Rat, Human

Product name

Anti-ATM antibody [EPR17059]
See all ATM primary antibodies
Description

Rabbit monoclonal [EPR17059] to ATM
Host species

Rabbit
Tested Applications & Species

Application Species
IP
Human

WB
Mouse

Rat

Human

See all applications and species data
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Rat testis, mouse testis and mouse spleen lysates; PC-12, 293 and SH-SY5Y whole cell lysates. ICC/IF: RAW 264.7 cells. IP: HEK-293 and SH-SY5Y whole cell lysates. Flow Cyt: NIH/3T3 (mouse embryo)
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IP	Human
WB	Mouse Rat Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR17059
Isotype

IgG
Research areas

Western blot - Anti-ATM antibody [EPR17059] (ab199726)

All lanes : Anti-ATM antibody [EPR17059] (ab199726) at 1/2000 dilution

Lane 1 : Rat testis lysate
Lane 2 : Mouse testis lysate
Lane 3 : 293 (Human epithelial cells from embryonic kidney) whole cell lysate
Lane 4 : SH-SY5Y (Human neuroblastoma from bone marrow cells) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 350 kDa
Observed band size: 350 kDa

Exposure time: 20 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-ATM antibody [EPR17059] (ab199726)

All lanes : Anti-ATM antibody [EPR17059] (ab199726) at 1/2000 dilution

Lane 1 : Rat testis lysate
Lane 2 : Mouse testis lysate
Lane 3 : Mouse spleen lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 350 kDa
Observed band size: 350 kDa

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-ATM antibody [EPR17059] (ab199726)

Anti-ATM antibody [EPR17059] (ab199726) at 1/5000 dilution + 293 (Human epithelial cells from embryonic kidney) whole cell lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 350 kDa
Observed band size: 350 kDa

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunoprecipitation - Anti-ATM antibody [EPR17059] (ab199726)

ATM was immunoprecipitated from 1mg of HEK-293 (Human epithelial cells from embryonic kidney) whole cell lysate with ab199726 at 1/100 dilution. Western blot was performed from the immunoprecipitate using ab199726 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
Lane 1: HEK-293 whole cell lysate 10ug (Input). Lane 2: ab199726 IP in HEK-293 whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab199726 in HEK-293 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds.
Immunoprecipitation - Anti-ATM antibody [EPR17059] (ab199726)

ATM was immunoprecipitated from 1mg of HEK-293 (Human epithelial cells from embryonic kidney) whole cell lysate with ab199726 at 1/100 dilution. Western blot was performed from the immunoprecipitate using ab199726 at 1/500 dilution (Panel A) or ab32420 at 1/500 dilution (Panel B). Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
Lane 1: HEK-293 whole cell lysate 10ug (Input). Lane 2: ab199726 IP in HEK-293 whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab199726 in HEK-293 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds (Panel A and B).
Immunoprecipitation - Anti-ATM antibody [EPR17059] (ab199726)

ATM was immunoprecipitated from 1mg of SH-SY5Y (Human neuroblastoma from bone marrow cells) whole cell lysate with ab199726 at 1/100 dilution. Western blot was performed from the immunoprecipitate using ab199726 at 1/500 dilution (Panel A) or ab32420 at 1/500 dilution (Panel B). Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
Lane 1: SH-SY5Y whole cell lysate 10ug (Input). Lane 2: ab199726 IP in SH-SY5Y whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab199726 in SH-SY5Y whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds (Panel A and B).
Anti-ATM antibody [EPR17059] (ab199726)

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