Human FADD Matched Antibody Pair Kit (ab217621)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Human
- Range: 250 pg/ml - 16000 pg/ml
Overview
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Product name
Human FADD Matched Antibody Pair Kit
See all FADD kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
92.2 pg/ml -
Range
250 pg/ml - 16000 pg/ml -
Species reactivity
Reacts with: Human -
Product overview
Human FADD Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant human FADD.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab214026. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
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Platform
Reagents
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests Human FADD Capture Antibody 2 x 50µg 1 x 50µg Human FADD Detector Antibody 2 x 12.5µg 1 x 12.5µg Human FADD Lyophilized Protein 2 vials 1 vial -
Research areas
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Function
Apoptotic adaptor molecule that recruits caspase-8 or caspase-10 to the activated Fas (CD95) or TNFR-1 receptors. The resulting aggregate called the death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation. Active caspase-8 initiates the subsequent cascade of caspases mediating apoptosis. -
Tissue specificity
Expressed in a wide variety of tissues, except for peripheral blood mononuclear leukocytes. -
Sequence similarities
Contains 1 death domain.
Contains 1 DED (death effector) domain. -
Domain
Contains a death domain involved in the binding of the corresponding domain within Fas receptor. - Information by UniProt
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Alternative names
- FADD
- FADD protein
- FADD_HUMAN
see all -
Database links
- Entrez Gene: 8772 Human
- Omim: 602457 Human
- SwissProt: Q13158 Human
- Unigene: 86131 Human
Images
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Human FADD standard curve.
Standard calibration curve. Background subtracted values are graphed.
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Sandwich ELISA - Human FADD Matched Antibody Pair Kit (ab217621)To learn more about the advantages of recombinant antibodies see here.
