Anti-VP16 tag antibody (ab4808)
Key features and details
- Rabbit polyclonal to VP16 tag
- Suitable for: WB, IHC-P, IHC-Fr
- Reacts with: Species independent
- Isotype: IgG
Overview
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Product name
Anti-VP16 tag antibody -
Description
Rabbit polyclonal to VP16 tag -
Host species
Rabbit -
Specificity
This antibody was raised against an immunogen that is predicted to react with HSV-1. We do not expect this product to react with HSV-2. Abcam welcomes customer feedback and would appreciate any comments regarding this product. -
Tested applications
Suitable for: WB, IHC-P, IHC-Frmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Synthetic peptide corresponding to Herpes simplex virus VP16 tag aa 450 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available asab13406) -
Positive control
- This antibody recognises GAL4-VP16 recombinant fusion protein (30kDa) in Western blot.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab4808 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB 1/500 - 1/2000. Detects a band of approximately 30 kDa. IHC-P Use at an assay dependent concentration. IHC-Fr Use at an assay dependent concentration. Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VP16 tag antibody (ab4808)
ab4808 at a 1/100 dilution staining mouse brain sections by Immunohistochemistry (Formalin-fixed paraffin-embedded sections). Following heat mediated antigen retrieval, the antibody was incubated with the tissue for 24 hours. Bound antibody was detected using an Alexa-Fluor 488 ® conjugated Donkey anti-Rabbit IgG.
This image is courtesy of an Abreview submitted on 10 March 2006.
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Western blot - Anti-VP16 tag antibody (ab4808)Lane 1 : Marker
Lanes 2-3 : Anti-VP16 tag antibody (ab4808) at 1 µg/ml
Lane 2 : GAL4-VP16 human recombinant E.coli
Lane 3 : GAL4-VP16 human recombinant E.coli
with VP16 tag peptide (ab13406) at 1 µg/ml
Secondary
Lanes 2-3 : Goat polyclonal to Rabbit IgG H&L (HRP) Pre-Adsorbed at 1/10000 dilution
Performed under reducing conditions.
Protocols
References (22)
ab4808 has been referenced in 22 publications.
- Rodrigue A et al. A global functional analysis of missense mutations reveals two major hotspots in the PALB2 tumor suppressor. Nucleic Acids Res 47:10662-10677 (2019). PubMed: 31586400
- Saito AN et al. Structure-function study of a novel inhibitor of the casein kinase 1 family in Arabidopsis thaliana. Plant Direct 3:e00172 (2019). PubMed: 31549020
- Wang L et al. Two Paralogous Tetraspanins TSP-12 and TSP-14 Function with the ADAM10 Metalloprotease SUP-17 to Promote BMP Signaling in Caenorhabditis elegans. PLoS Genet 13:e1006568 (2017). PubMed: 28068334
- Fehrenbacher N et al. The G protein-coupled receptor GPR31 promotes membrane association of KRAS. J Cell Biol 216:2329-2338 (2017). PubMed: 28619714
- Smith RWP et al. Viral and cellular mRNA-specific activators harness PABP and eIF4G to promote translation initiation downstream of cap binding. Proc Natl Acad Sci U S A 114:6310-6315 (2017). PubMed: 28559344
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VP16 tag antibody (ab4808)
ab4808 at a 1/100 dilution staining mouse brain sections by Immunohistochemistry (Formalin-fixed paraffin-embedded sections). Following heat mediated antigen retrieval, the antibody was incubated with the tissue for 24 hours. Bound antibody was detected using an Alexa-Fluor 488 ® conjugated Donkey anti-Rabbit IgG.
This image is courtesy of an Abreview submitted on 10 March 2006.
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Western blot - Anti-VP16 tag antibody (ab4808)Lane 1 : Marker
Lanes 2-3 : Anti-VP16 tag antibody (ab4808) at 1 µg/ml
Lane 2 : GAL4-VP16 human recombinant E.coli
Lane 3 : GAL4-VP16 human recombinant E.coli
with VP16 tag peptide (ab13406) at 1 µg/ml
Secondary
Lanes 2-3 : Goat polyclonal to Rabbit IgG H&L (HRP) Pre-Adsorbed at 1/10000 dilution
Performed under reducing conditions.