Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CLEC4C antibody [EPR21981] (ab239077)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21981] to CLEC4C
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-CLEC4C antibody [EPR21981]
    See all CLEC4C primary antibodies

  • Description

    Rabbit monoclonal [EPR21981] to CLEC4C

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment within Human CLEC4C aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: Q8WTT0

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: Human tonsil lysate. IHC-P: Human tonsil tissue.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR21981

  • Isotype

    IgG

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLEC4C antibody [EPR21981] (ab239077)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLEC4C antibody [EPR21981] (ab239077)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CLEC4C with ab239077 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counter stained with hematoxylin. Membranous staining in plasmacytoid dendritic cells (PDCs) of human tonsil (PMID:23847189, PMID:11748283) is observed.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLEC4C antibody [EPR21981] (ab239077)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLEC4C antibody [EPR21981] (ab239077)

    Immunohistochemical analysis of paraffin-embedded human colon tissue labeling CLEC4C with ab239077 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counter stained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Negative control: Negative staining in human colon (PMID:11748283).

     

  • Western blot - Anti-CLEC4C antibody [EPR21981] (ab239077)
    Western blot - Anti-CLEC4C antibody [EPR21981] (ab239077)
    All lanes : Anti-CLEC4C antibody [EPR21981] (ab239077) at 1/1000 dilution

    Lane 1 : Human tonsil lysate
    Lane 2 : Human tonsil lysate treated with PNGase F

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

    Predicted band size: 25 kDa
    Observed band size: 25,37 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking and dilution buffer: 5% NFDM/TBST.

    The molecular mass at 37-kDa is the glycosylated form, consistent with what has been described in the literature (PMID:16998465). The 25-kDa band (lane 2) was observed upon deglycosylation.

    This blot was developed using a higher sensitivity ECL substrate

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