Anti-ATM antibody (ab226985)
Key features and details
- Rabbit polyclonal to ATM
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ATM antibody
See all ATM primary antibodies -
Description
Rabbit polyclonal to ATM -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Synthetic peptide within Human ATM (internal sequence). The exact sequence is proprietary. (Carrier-protein conjugated).
Database link: Q13315 -
Positive control
- WB: HeLa and H1299 whole cell extracts. ICC/IF: HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ATM antibody (ab226985)All lanes : Anti-ATM antibody (ab226985) at 1/500 dilution
Lane 1 : Non-transfected HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 2 : HeLa, transfected with ATM shRNA, whole cell extract
Lysates/proteins at 60 µg per lane.
Predicted band size: 350 kDa
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Western blot - Anti-ATM antibody (ab226985)Anti-ATM antibody (ab226985) at 1/1000 dilution + H1299 whole cell extract at 30 µg
Predicted band size: 350 kDa5% SDS-PAGE gel.
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Immunocytochemistry/ Immunofluorescence - Anti-ATM antibody (ab226985)4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for ATM (green) using ab226985 at 1/500 dilution in ICC/IF.
Red: phalloidin, a cytoskeleton marker, at 1/200 dilution.
