Antibodies, Proteins, Kits and Reagents for Life Science

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [SP224] to ATM
Suitable for: ICC/IF, Flow Cyt, IHC-P
Reacts with: Human

Product name

Anti-ATM antibody [SP224]
See all ATM primary antibodies
Description

Rabbit monoclonal [SP224] to ATM
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- Human prostate adenocarcinoma tissue; HeLa cells. ICC/IF: HeLa cells Flow Cyt: HeLa cells
General notes
FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.1% Sodium azide
Constituents: 1% BSA, PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

SP224
Isotype

IgG
Research areas

Immunocytochemistry/ Immunofluorescence - Anti-ATM antibody [SP224] (ab183324)

Immunocytochemistry/ Immunofluorescence analysis of HeLa (human cervix adenocarcinoma epithelial cell) cells labeling ATM with purified ab183324 at 1/200 (10 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Flow Cytometry - Anti-ATM antibody [SP224] (ab183324)

Flow cytometry analysis of Hela (human cervix adenocarcinoma) labeling ATM with purified ab183324 at 1/2100 dilution (1.00 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotype control - Rabbit monoclonal IgG (ab172730) (Black). Unlableled control - Unlabelled cells (blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATM antibody [SP224] (ab183324)

Immunohistochemical analysis of paraffin embedded Human prostate adenocarcinoma tissue labeling ATM with ab183324 at 1/100.
Flow Cytometry - Anti-ATM antibody [SP224] (ab183324)

Flow cytometric analysis of ATM in HeLa cells using ab183324 at 1/100 (green) compared to negative control of rabbit IgG (blue).
Anti-ATM antibody [SP224] (ab183324)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com