Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ATM antibody [EPR17059] - BSA and Azide free (ab232588)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17059] to ATM - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-ATM antibody [EPR17059] - BSA and Azide free
    See all ATM primary antibodies

  • Description

    Rabbit monoclonal [EPR17059] to ATM - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt or ICC/IF

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab232588 is the carrier-free version of ab199726. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab232588 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunoprecipitation - Anti-ATM antibody [EPR17059] - BSA and Azide free (ab232588)
    Immunoprecipitation - Anti-ATM antibody [EPR17059] - BSA and Azide free (ab232588)

    ATM was immunoprecipitated from 1mg of HEK-293 (Human epithelial cells from embryonic kidney) whole cell lysate with ab199726 at 1/100 dilution. Western blot was performed from the immunoprecipitate using ab199726 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

    Lane 1: HEK-293 whole cell lysate 10ug (Input). Lane 2: ab199726 IP in HEK-293 whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab199726 in HEK-293 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab199726).

  • Immunoprecipitation - Anti-ATM antibody [EPR17059] - BSA and Azide free (ab232588)
    Immunoprecipitation - Anti-ATM antibody [EPR17059] - BSA and Azide free (ab232588)

    ATM was immunoprecipitated from 1mg of HEK-293 (Human epithelial cells from embryonic kidney) whole cell lysate with ab199726 at 1/100 dilution. Western blot was performed from the immunoprecipitate using ab199726 at 1/500 dilution (Panel A) or ab32420 at 1/500 dilution (Panel B). Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

    Lane 1: HEK-293 whole cell lysate 10ug (Input). Lane 2: ab199726 IP in HEK-293 whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab199726 in HEK-293 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 30 seconds (Panel A and B).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab199726).

  • Immunoprecipitation - Anti-ATM antibody [EPR17059] - BSA and Azide free (ab232588)
    Immunoprecipitation - Anti-ATM antibody [EPR17059] - BSA and Azide free (ab232588)

    ATM was immunoprecipitated from 1mg of SH-SY5Y (Human neuroblastoma from bone marrow cells) whole cell lysate with ab199726 at 1/100 dilution. Western blot was performed from the immunoprecipitate using ab199726 at 1/500 dilution (Panel A) or ab32420 at 1/500 dilution (Panel B). Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

    Lane 1: SH-SY5Y whole cell lysate 10ug (Input). Lane 2: ab199726 IP in SH-SY5Y whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab199726 in SH-SY5Y whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 30 seconds (Panel A and B).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab199726).

  • Anti-ATM antibody [EPR17059] - BSA and Azide free (ab232588)
    Anti-ATM antibody [EPR17059] - BSA and Azide free (ab232588)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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