Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ATM (phospho S1981) antibody [EP1890Y] - BSA and Azide free (ab208775)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1890Y] to ATM (phospho S1981) - BSA and Azide free
  • Suitable for: WB, Flow Cyt, Dot blot, IHC-P, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-ATM (phospho S1981) antibody [EP1890Y] - BSA and Azide free
    See all ATM primary antibodies

  • Description

    Rabbit monoclonal [EP1890Y] to ATM (phospho S1981) - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cyt, Dot blot, IHC-P, IPmore details
    Unsuitable for: ICC/IF

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK293 cell lysate treated with doxorubicin. IHC-P: Human gastric carcinoma, breast carcinoma, tonsil, cervical carcinoma, hepatocellular carcinoma and endometrial carcinoma tissues and mouse endometrium tissue. ICC/IF: HepG2 and HEK293 cells. IP: HEK293 cell lysate treated with doxorubicin.

  • General notes

    ab208775 is the carrier-free version of ab81292 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab208775 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-ATM (phospho S1981) antibody [EP1890Y] - BSA and Azide free (ab208775)
    Western blot - Anti-ATM (phospho S1981) antibody [EP1890Y] - BSA and Azide free (ab208775)
    Anti-ATM (phospho S1981) antibody [EP1890Y] - BSA and Azide free (ab208775) + HEK293 (human embryonic kidney) treated with Doxorubicin whole cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051)

    Predicted band size: 351 kDa
    Observed band size: 370 kDa
    why is the actual band size different from the predicted?


    Exposure time: 1 minute


    Blocking buffer and concentration: 5% NFDM/TBST

    Diluting buffer and concentration: 5% NFDM/TBST

  • Immunoprecipitation - Anti-ATM (phospho S1981) antibody [EP1890Y] - BSA and Azide free (ab208775)
    Immunoprecipitation - Anti-ATM (phospho S1981) antibody [EP1890Y] - BSA and Azide free (ab208775)

    ATM was immunoprecipitated from HEK-293 (Human embryonic kidney epithelial cell) treated with Doxorubicin whole cell lysate with ab81292 at 1/30 dilution (5 µg in 1 mg lysates). Western blot was performed from the immunoprecipitate using ab81292 at 1/2000 dilution. An anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG was used as secondary antibody at 1/1500 dilution.
    Lane 1: HEK-293 treated with Doxorubicin whole cell lysate 10 µg (Input).
    Lane 2: ab81292 IP in HEK-293 treated with Doxorubicin whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab81292 in HEK-293 treated with Doxorubicin whole cell lysate.
    Blocking/Dilution buffer: 5% NFDM/TBST.
    Exposure time: 3 minutes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab81292).

  • Flow Cytometry - Anti-ATM (phospho S1981) antibody [EP1890Y] - BSA and Azide free (ab208775)
    Flow Cytometry - Anti-ATM (phospho S1981) antibody [EP1890Y] - BSA and Azide free (ab208775)

    Flow Cytometry analysis of HepG2 (Human hepatocellular carcinoma) cells labeling ATM (phospho S1981) with purified ab81292 at 1/60 dilution (10 µg/mL) (red).

    Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor®488) at 1/2000 dilution was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab81292).

  • Dot Blot - Anti-ATM (phospho S1981) antibody [EP1890Y] - BSA and Azide free (ab208775)
    Dot Blot - Anti-ATM (phospho S1981) antibody [EP1890Y] - BSA and Azide free (ab208775)

    Dot blot analysis of ATM peptides using ab81292 at 1/000 dilution followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody (ab97051) at 1/100,000 dilution.

    Blocking and diluting buffer was 5% NFDM/TBST, exposure time 3 minutes.

    Lane 1: ATM (pS1981) phospho peptide

    Lane 2: ATM non-phospho peptide

    Lane 3: ATM (pS428) phospho peptide

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab81292).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATM (phospho S1981) antibody [EP1890Y] - BSA and Azide free (ab208775)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATM (phospho S1981) antibody [EP1890Y] - BSA and Azide free (ab208775)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocellular carcinoma tissue labelling ATM (phospho S1981) with purified ab81292 at 1/70. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, an HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500).

    Negative control using PBS instead of primary antibody.

    Counterstained with hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab81292).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATM (phospho S1981) antibody [EP1890Y] - BSA and Azide free (ab208775)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATM (phospho S1981) antibody [EP1890Y] - BSA and Azide free (ab208775)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human gastric carcinoma tissue labeling ATM with unpurified ab81292 at a 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab81292).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATM (phospho S1981) antibody [EP1890Y] - BSA and Azide free (ab208775)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATM (phospho S1981) antibody [EP1890Y] - BSA and Azide free (ab208775)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labeling ATM with unpurified ab81292.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab81292).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATM (phospho S1981) antibody [EP1890Y] - BSA and Azide free (ab208775)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATM (phospho S1981) antibody [EP1890Y] - BSA and Azide free (ab208775)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of normal human tonsil tissue labeling ATM with unpurified ab81292.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab81292).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATM (phospho S1981) antibody [EP1890Y] - BSA and Azide free (ab208775)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATM (phospho S1981) antibody [EP1890Y] - BSA and Azide free (ab208775)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cervical carcinoma tissue labeling ATM with unpurified ab81292.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab81292).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATM (phospho S1981) antibody [EP1890Y] - BSA and Azide free (ab208775)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATM (phospho S1981) antibody [EP1890Y] - BSA and Azide free (ab208775)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human endometrial carcinoma tissue labeling ATM with unpurified ab81292.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab81292).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-ATM (phospho S1981) antibody [EP1890Y] - BSA and Azide free (ab208775)
    Anti-ATM (phospho S1981) antibody [EP1890Y] - BSA and Azide free (ab208775)

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