Antibodies, Proteins, Kits and Reagents for Life Science

Anti-ATM antibody [SP224] - BSA and Azide free (ab213522)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [SP224] to ATM - BSA and Azide free
Suitable for: Flow Cyt, IHC-P, ICC/IF
Reacts with: Human

Product name

Anti-ATM antibody [SP224] - BSA and Azide free
See all ATM primary antibodies
Description

Rabbit monoclonal [SP224] to ATM - BSA and Azide free
Host species

Rabbit
Tested applications

Suitable for: Flow Cyt, IHC-P, ICC/IFmore details
Species reactivity

Reacts with: Human
Predicted to work with: Mouse, Pig
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- IHC-P: Human prostate adenocarcinoma Tissue ICC/IF: HeLa cells Flow Cyt: HeLa cells
General notes
FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Ab213522 is the carrier-free version of ab183324. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab213522 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.20
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A/G purified
Purification notes

Purified from TCS by protein A/G.
Clonality

Monoclonal
Clone number

SP224
Isotype

IgG
Research areas

Immunocytochemistry/ Immunofluorescence - Anti-ATM antibody [SP224] - BSA and Azide free (ab213522)

Immunocytochemistry/ Immunofluorescence analysis of HeLa (human cervix adenocarcinoma epithelial cell) cells labeling ATM with purified ab183324 at 1/200 (10 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183324).
Flow Cytometry - Anti-ATM antibody [SP224] - BSA and Azide free (ab213522)

Flow cytometry analysis of HeLa (human cervix adenocarcinoma) labeling ATM with purified ab183324 at 1/2100 dilution (1.00 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotypecontrol - Rabbit monoclonal IgG (ab172730) (black). Unlableled control - Unlabelled cells (blue).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183324).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATM antibody [SP224] - BSA and Azide free (ab213522)

Immunohistochemical analysis of paraffin embedded human prostate adenocarcinoma tissue labeling ATM with ab183324 at 1/100.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab183324).
Flow Cytometry - Anti-ATM antibody [SP224] - BSA and Azide free (ab213522)

Flow cytometric analysis of ATM in HeLa cells using ab183324 at 1/100 (green) compared to negative control of rabbit IgG (blue).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183324).
Anti-ATM antibody [SP224] - BSA and Azide free (ab213522)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com