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Anti-ATM (phospho S1981) antibody [10H11.E12] (ab36810)

Key features and details

  • Mouse monoclonal [10H11.E12] to ATM (phospho S1981)
  • Suitable for: Flow Cyt, WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-ATM (phospho S1981) antibody [10H11.E12]
    See all ATM primary antibodies

  • Description

    Mouse monoclonal [10H11.E12] to ATM (phospho S1981)

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to Human ATM.
    Database link: Q13315

  • Positive control

    • Irradiated normal human fibroblasts (no reactivity against non-irradiated cell extracts).

  • General notes

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATM (phospho S1981) antibody [10H11.E12] (ab36810)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATM (phospho S1981) antibody [10H11.E12] (ab36810)

    ab36810 staining human colon tissue. Staining is localized to the nucleus.
    Left panel: with primary antibody at 4 µg/ml. Right panel: Isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus at room temperature. Sections were rehydrated and antigen retrieved with the DAKO 3-in-1 antigen retrieval buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with hematoxylin and coverslipped under DePeX.

    Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

  • Western blot - Anti-ATM (phospho S1981) antibody [10H11.E12] (ab36810)
    Western blot - Anti-ATM (phospho S1981) antibody [10H11.E12] (ab36810)
    All lanes : Anti-ATM (phospho S1981) antibody [10H11.E12] (ab36810) at 10 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Extract from patient with Ataxia-Telangiectasia whole cell lysate
    Lane 3 : Irradiated HeLa Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Lanes 1 & 3 : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Lane 2 : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under non-reducing conditions.

    Predicted band size: 370 kDa
    Observed band size: 370 kDa
    Additional bands at: 100 kDa, 110 kDa, 145 kDa, 200 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 20 minutes
  • Flow Cytometry - Anti-ATM (phospho S1981) antibody [10H11.E12] (ab36810)
    Flow Cytometry - Anti-ATM (phospho S1981) antibody [10H11.E12] (ab36810)

    Overlay histogram showing HeLa cells stained with ab36810 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab36810, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • Western blot - Anti-ATM (phospho S1981) antibody [10H11.E12] (ab36810)
    Western blot - Anti-ATM (phospho S1981) antibody [10H11.E12] (ab36810)
    All lanes : Anti-ATM (phospho S1981) antibody [10H11.E12] (ab36810) at 1/1000 dilution

    Lane 1 : Control lane
    Lane 2 : Irradiated Human fibroblasts (10 Gy gamma-irradiation)
    Lane 3 : Molecular weight marker
    Lane 4 : Peroxidated Human fibroblasts (300 µM hydrogen peroxide)
    Lane 5 : Peroxidated Human fibroblasts (1 mM hydrogen peroxide)
    Lane 6 : Peroxidated Human fibroblasts (10 mM hydrogen peroxide)

    Developed using the ECL technique.

    Predicted band size: 370 kDa
    Observed band size: 370 kDa



    Lysate loading concentration: 40µg

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