Anti-KIF22 antibody [EP2747Y] (ab75783)
![Anti-KIF22 antibody [EP2747Y] (ab75783)](https://www.abcam.com/ps/products/75/ab75783/Images/ab75783-450437-anti-kif22-antibody-ep2747y-western-blot-wildtype-hek293t-kif22-knockout-hek293t.jpg "Anti-KIF22 antibody [EP2747Y] (ab75783)")
Key features and details
- Rabbit monoclonal [EP2747Y] to KIF22
- Suitable for: WB
- Knockout validated
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-KIF22 antibody [EP2747Y]
See all KIF22 primary antibodies -
Description
Rabbit monoclonal [EP2747Y] to KIF22 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide within Human KIF22 (N terminal). The exact sequence is proprietary.
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Positive control
- HEK-293T and HeLa whole cell lysate (ab150035)
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP2747Y -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-KIF22 antibody [EP2747Y] (ab75783)All lanes : Anti-KIF22 antibody [EP2747Y] (ab75783) at 1/1000 dilution
Lane 1 : Wild-type HEK-293T cell lysate
Lane 2 : KIF22 knockout HEK-293T cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 73 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?Lanes 1 - 2: Merged signal (red and green). Green - ab75783 observed at 75 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.
ab75783 was shown to react with KIF22 in HEK-293T wild-type cells in western blot with loss of signal observed in KIF22 knockout cell line ab266817 (KIF22 knockout cell lysate ab258018). Wild-type and KIF22 knockout HEK-293T cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab75783 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-KIF22 antibody [EP2747Y] (ab75783)](https://www.abcam.com/ps/products/75/ab75783/Images/ab75783-90864-anti-kif22-antibody-ep2747y-western-blot.jpg)
Western blot - Anti-KIF22 antibody [EP2747Y] (ab75783)Anti-KIF22 antibody [EP2747Y] (ab75783) at 1/5000 dilution + HeLa cell
lysate at 10 µg
Secondary
HRP labelled oat anti rabbit at 1/2000 dilution
Predicted band size: 73 kDa
Observed band size: 73 kDa
