Anti-KIF18A antibody (ab72417)
Key features and details
- Rabbit polyclonal to KIF18A
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-KIF18A antibody
See all KIF18A primary antibodies -
Description
Rabbit polyclonal to KIF18A -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
-
Immunogen
Synthetic peptide corresponding to a region between residue 848 and the C-terminus (residue 898) of human KIF18A (NP_112494.2)
-
Positive control
- HeLa and 293T whole cell lysates.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab72417 was affinity purified using an epitope specific to KIF18A immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-KIF18A antibody (ab72417)All lanes : Anti-KIF18A antibody (ab72417) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 101 kDa
Observed band size: 117 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes
-
Immunoprecipitation - Anti-KIF18A antibody (ab72417)Detection of KIF18A by Western Blot of Immunprecipitate.
ab72417 at 1µg/ml staining KIF18A in HeLa whole cell lysate immunoprecipitated using ab72417 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 30 seconds.
