Human USP7 (HAUSP) knockout HEK-293T cell lysate (ab257284)
Properties
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Storage instructions
Store at -80°C. Please refer to protocols. -
Components 1 kit ab260944 - Human USP7 knockout HEK293T cell lysate 1 x 100µg ab255553 - Human wild-type HEK293T cell lysate 1 x 100µg -
Research areas
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Cell type
epithelial -
STR Analysis
Amelogenin X D5S818: 8, 9 D13S317: 12, 14 D7S820: 11 D16S539: 9, 13 vWA: 16, 19 TH01: 7, 9.3 TPOX: 11 CSF1PO: 11, 12
Images
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Western blot - Human USP7 (HAUSP) knockout HEK293T cell lysate (ab257284)Lane 1: Wild-type HEK-293T cell lysate (20µg)
Lane 2: USP7 knockout HEK-293T cell lysate (20µg)
Lanes 1- 2: Merged signal (red and green). Green - ab109109 observed at 128 kDa. Red - loading control, ab8245 observed at 37 kDa.
ab109109 Anti-HAUSP / USP7 antibody [EPR4254] was shown to specifically react with HAUSP / USP7 in wild-type HEK-293T cells in western blot. The band observed in the knockout cell line ab266535 (knockout cell lysate ab257284) lane below 128kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type and HAUSP / USP7 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab109109 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging. -
Western blot - Human USP7 (HAUSP) knockout HEK293T cell lysate (ab257284)Lane 1: Wild-type HEK-293T cell lysate (20µg)
Lane 2: USP7 knockout HEK-293T cell lysate (20µg)
Lanes 1- 2: Merged signal (red and green). Green - ab108931 observed at 128 kDa. Red - loading control, ab8245 observed at 37 kDa.
ab108931 Anti-HAUSP / USP7 antibody [EPR4253] was shown to specifically react with HAUSP / USP7 in wild-type HEK-293T cells in western blot. The band observed in the knockout cell line ab266535 (knockout cell lysate ab257284) lane below 128kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type and HAUSP / USP7 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab108931 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging. -
Sanger Sequencing - Human USP7 knockout HEK293T cell lysate (ab257284)Allele-1: 1 bp insertion in exon2
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Sanger Sequencing - Human USP7 knockout HEK293T cell lysate (ab257284)Allele-2: Insertion of the selection cassette in exon2