Anti-Nup153 antibody [R4C8] (ab81463)
![Anti-Nup153 antibody [R4C8] (ab81463)](https://www.abcam.com/ps/products/81/ab81463/Images/ab81463-96660-anti-nup153-antibody-r4c8-western-blot.jpg "Anti-Nup153 antibody [R4C8] (ab81463)")
Key features and details
- Rat monoclonal [R4C8] to Nup153
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG2a
Overview
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Product name
Anti-Nup153 antibody [R4C8]
See all Nup153 primary antibodies -
Description
Rat monoclonal [R4C8] to Nup153 -
Host species
Rat -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment, corresponding to amino acids 610-1191 of Rat Nup153, fused to a tag.
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Epitope
Amino acids 610-1191 (Zn finger and FG repeats domain). -
Positive control
- WB: Nuclear membrane fraction of HeLa cells and NIH3T3 cells. ICC/IF: HeLa cells and rat neuron.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Ion Exchange Chromatography -
Purification notes
This antibody was purified from the serum free cultured medium of the hybridoma under mild conditions by propriety chromatography processes. It is filter sterilized. -
Clonality
Monoclonal -
Clone number
R4C8 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Images
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Western blot - Anti-Nup153 antibody [R4C8] (ab81463)All lanes : Anti-Nup153 antibody [R4C8] (ab81463)
Lane 1 : Nuclear membrane fraction from HeLa cells
Lane 2 : Nuclear membrane fraction from NIH3T3 cells
Predicted band size: 154 kDa
Observed band size: 160 kDa why is the actual band size different from the predicted?
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![Immunocytochemistry/ Immunofluorescence - Anti-Nup153 antibody [R4C8] (ab81463)](https://www.abcam.com/ps/products/81/ab81463/Images/ab81463-96659-anti-nup153-antibody-r4c8-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Nup153 antibody [R4C8] (ab81463)Immunofluorescent staining of HeLa cells with ab81463. -
![Immunocytochemistry/ Immunofluorescence - Anti-Nup153 antibody [R4C8] (ab81463)](https://www.abcam.com/ps/products/81/ab81463/Images/ab81463-96658-anti-nup153-antibody-r4c8-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Nup153 antibody [R4C8] (ab81463)Immunofluorescent staining of rat neuron with ab81463. The dots are Nup153.
