Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TR4 antibody [EPR1773(2)] (ab109301)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1773(2)] to TR4
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-TR4 antibody [EPR1773(2)]
    See all TR4 primary antibodies

  • Description

    Rabbit monoclonal [EPR1773(2)] to TR4

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Rat
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • PC3 cell lysate, 293T cell lysate; Human testis tissue

  • General notes

     

     

    Previously labelled as NR2C2. 

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR1773(2)

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-TR4 antibody [EPR1773(2)] (ab109301)
    Western blot - Anti-TR4 antibody [EPR1773(2)] (ab109301)
    All lanes : Anti-TR4 antibody [EPR1773(2)] (ab109301) at 1/1000 dilution

    Lane 1 : Wild-type HEK293T cell lysate
    Lane 2 : NR2C2 knockout HEK293T cell lysate
    Lane 3 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 65 kDa
    Observed band size: 67 kDa
    why is the actual band size different from the predicted?



    Lanes 1-3: Merged signal (red and green). Green - ab109301 observed at 67 kDa. Red - loading control ab8245 observed at 36 kDa.

     ab109301 Anti-TR4 antibody [EPR1773(2)] was shown to specifically react with TR4 in wild-type HEK293T cells. The band observed in knockout cell line ab266228 (knockout cell lysate ab257563) lane below 67 kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type and TR4 knockout samples were subjected to SDS-PAGE. ab109301 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunoprecipitation - Anti-TR4 antibody [EPR1773(2)] (ab109301)
    Immunoprecipitation - Anti-TR4 antibody [EPR1773(2)] (ab109301)

    ab109301 (purified) at 1/30 immunoprecipitating TR4 in 10 μg HEK293 cell lysate (Lanes 1 and 2, observed at 67 kDa). Lane 3 - Rabbit monoclonal IgG (ab172730). For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution. Blocking buffer and concentration: 5% NFDM/TBST Dilution buffer and concentration: 5% NFDM/TBST

  • Western blot - Anti-TR4 antibody [EPR1773(2)] (ab109301)
    Western blot - Anti-TR4 antibody [EPR1773(2)] (ab109301)
    All lanes : Anti-TR4 antibody [EPR1773(2)] (ab109301) at 1/5000 dilution (purified)

    Lane 1 : PC-12 whole cell lysate
    Lane 2 : 3T3-L1 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 65 kDa
    Observed band size: 67 kDa why is the actual band size different from the predicted?



    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • Western blot - Anti-TR4 antibody [EPR1773(2)] (ab109301)
    Western blot - Anti-TR4 antibody [EPR1773(2)] (ab109301)
    Anti-TR4 antibody [EPR1773(2)] (ab109301) at 1/5000 dilution (purified) + HEK293 whole cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 65 kDa
    Observed band size: 67 kDa why is the actual band size different from the predicted?



    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • Western blot - Anti-TR4 antibody [EPR1773(2)] (ab109301)
    Western blot - Anti-TR4 antibody [EPR1773(2)] (ab109301)
    Anti-TR4 antibody [EPR1773(2)] (ab109301) at 1/2000 dilution (purified) + PC-3 whole cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 65 kDa
    Observed band size: 67 kDa why is the actual band size different from the predicted?

  • Western blot - Anti-TR4 antibody [EPR1773(2)] (ab109301)
    Western blot - Anti-TR4 antibody [EPR1773(2)] (ab109301)
    All lanes : Anti-TR4 antibody [EPR1773(2)] (ab109301) at 1/1000 dilution (Unpurified)

    Lane 1 : PC3 cell lysate
    Lane 2 : 293T cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 65 kDa
    Observed band size: 67 kDa why is the actual band size different from the predicted?

  • Anti-TR4 antibody [EPR1773(2)] (ab109301)
    Anti-TR4 antibody [EPR1773(2)] (ab109301)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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