Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-NR2F6/EAR-2 antibody [EPR22260] - BSA and Azide free (ab242018)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22260] to NR2F6/EAR-2 - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), IHC-P, WB, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-NR2F6/EAR-2 antibody [EPR22260] - BSA and Azide free
    See all NR2F6/EAR-2 primary antibodies

  • Description

    Rabbit monoclonal [EPR22260] to NR2F6/EAR-2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), IHC-P, WB, IPmore details
    Unsuitable for: ICC/IF

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human kidney and human breast carcinoma tissues. Flow Cyt (intra): HeLa cells. IP: HeLa whole cell lysate.

  • General notes

    ab242018 is the carrier-free version of ab223265.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NR2F6/EAR-2 antibody [EPR22260] - BSA and Azide free (ab242018)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NR2F6/EAR-2 antibody [EPR22260] - BSA and Azide free (ab242018)

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labeling NR2F6/EAR-2 with ab223265 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on human breast carcinoma (PMID: 27775588). Counterstained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223265).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NR2F6/EAR-2 antibody [EPR22260] - BSA and Azide free (ab242018)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NR2F6/EAR-2 antibody [EPR22260] - BSA and Azide free (ab242018)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling NR2F6/EAR-2 with ab223265 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on human kidney (PMID: 10713182). Counterstained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223265).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-NR2F6/EAR-2 antibody [EPR22260] - BSA and Azide free (ab242018)
    Immunoprecipitation - Anti-NR2F6/EAR-2 antibody [EPR22260] - BSA and Azide free (ab242018)

    NR2F6/EAR-2 was immunoprecipitated from 0.35 mg HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab223265 at 1/30 dilution. Western blot was performed on the immunoprecipitate using ab223265 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/5000 dilution.

    Lane 1: HeLa whole cell lysate 10 µg (input).
    Lane 2: ab223265 IP in HeLa whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab223265 in HeLa whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 30 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223265).

  • Flow Cytometry - Anti-NR2F6/EAR-2 antibody [EPR22260] - BSA and Azide free (ab242018)
    Flow Cytometry - Anti-NR2F6/EAR-2 antibody [EPR22260] - BSA and Azide free (ab242018)

    Flow cytometric analysis of 4% paraformaldehyde-fixed 90% methanol permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling NR2F6/EAR-2 with ab223265 at 1/40 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223265).

  • Anti-NR2F6/EAR-2 antibody [EPR22260] - BSA and Azide free (ab242018)
    Anti-NR2F6/EAR-2 antibody [EPR22260] - BSA and Azide free (ab242018)

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