Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-NR2F6/EAR-2 antibody [EPR22260] (ab223265)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22260] to NR2F6/EAR-2
  • Suitable for: Flow Cyt, IP, WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-NR2F6/EAR-2 antibody [EPR22260]
    See all NR2F6/EAR-2 primary antibodies

  • Description

    Rabbit monoclonal [EPR22260] to NR2F6/EAR-2

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, HCT116, HT-29, HEK-293T and HepG2 whole cell lysates. IHC-P: Human kidney and human breast carcinoma tissues. Flow Cyt: HeLa cells. IP: HeLa whole cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR22260

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-NR2F6/EAR-2 antibody [EPR22260] (ab223265)
    Western blot - Anti-NR2F6/EAR-2 antibody [EPR22260] (ab223265)
    All lanes : Anti-NR2F6/EAR-2 antibody [EPR22260] (ab223265) at 1/1000 dilution

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma), whole cell lysate
    Lane 2 : HCT 116 (human colorectal carcinoma cell line), whole cell lysate
    Lane 3 : HT-29 (human colorectal adenocarcinoma cell line), whole cell lysate
    Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen), whole cell lysate
    Lane 5 : HepG2 (human liver hepatocellular carcinoma cell line), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 43 kDa
    Observed band size: 43 kDa


    Exposure time: 3 minutes


    Blocking/Diluting buffer and concentration: 5% NFDM/TBST 

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NR2F6/EAR-2 antibody [EPR22260] (ab223265)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NR2F6/EAR-2 antibody [EPR22260] (ab223265)

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labeling NR2F6/EAR-2 with ab223265 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on human breast carcinoma (PMID: 27775588). Counterstained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NR2F6/EAR-2 antibody [EPR22260] (ab223265)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NR2F6/EAR-2 antibody [EPR22260] (ab223265)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling NR2F6/EAR-2 with ab223265 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on human kidney (PMID: 10713182). Counterstained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

     

  • Immunoprecipitation - Anti-NR2F6/EAR-2 antibody [EPR22260] (ab223265)
    Immunoprecipitation - Anti-NR2F6/EAR-2 antibody [EPR22260] (ab223265)

    NR2F6/EAR-2 was immunoprecipitated from 0.35 mg HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab223265 at 1/30 dilution. Western blot was performed on the immunoprecipitate using ab223265 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/5000 dilution.

    Lane 1: HeLa whole cell lysate 10 µg (input).
    Lane 2: ab223265 IP in HeLa whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab223265 in HeLa whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 30 seconds.

  • Flow Cytometry - Anti-NR2F6/EAR-2 antibody [EPR22260] (ab223265)
    Flow Cytometry - Anti-NR2F6/EAR-2 antibody [EPR22260] (ab223265)

    Flow cytometric analysis of 4% paraformaldehyde-fixed 90% methanol permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling NR2F6/EAR-2 with ab223265 at 1/40 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Anti-NR2F6/EAR-2 antibody [EPR22260] (ab223265)
    Anti-NR2F6/EAR-2 antibody [EPR22260] (ab223265)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-NR2F6/EAR-2 antibody [EPR22260] (ab223265)

© 2021 Astana Biomed Group LLP. All rights reserved.