Antibodies, Proteins, Kits and Reagents for Life Science

526 012 ₸ Product size

100 µg 526 012 ₸ 1 mg 4 690 ₸

Order now and get it on Tuesday March 09, 2021

Anti-Histone H2A (acetyl K5) antibody [EP856Y] - BSA and Azide free (ab247302)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP856Y] to Histone H2A (acetyl K5) - BSA and Azide free
Suitable for: IP, ICC/IF, IHC-P, WB
Reacts with: Mouse, Rat, Human

Product name

Anti-Histone H2A (acetyl K5) antibody [EP856Y] - BSA and Azide free
See all Histone H2A primary antibodies
Description

Rabbit monoclonal [EP856Y] to Histone H2A (acetyl K5) - BSA and Azide free
Host species

Rabbit
Tested applications

Suitable for: IP, ICC/IF, IHC-P, WBmore details
Species reactivity

Reacts with: Mouse, Rat, Human
Predicted to work with: Caenorhabditis elegans
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
General notes
Ab247302 is the carrier-free version of ab45152. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab247302 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.2
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EP856Y
Isotype

IgG

Immunocytochemistry - Anti-Histone H2A (acetyl K5) antibody [EP856Y] - BSA and Azide free (ab247302)

This data was developed using ab45152, the same antibody clone in a different buffer formulation.
ab45152 staining Histone H2A (acetyl K5) in C6 (rat glioma) cells, treated and untreated with TSA,500ng/ml for 4 hours by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/200. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody. ab7291 and ab150120 were used as counterstains for primary antibody ab75748 and secondary antibody ab150077 respectively and DAPI was used as a nuclear counterstain. Negative control 1: Rabbit primary antibody and anti-mouse secondary antibody (ab150120)
Negative control 2: Mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab150077)
Immunoprecipitation - Anti-Histone H2A (acetyl K5) antibody [EP856Y] - BSA and Azide free (ab247302)

This data was developed using ab45152, the same antibody clone in a different buffer formulation.
ab45152 immunoprecipitating Histone H2A. 10µg of cell lysate was incubated with primary antibody at a dilution of 1/20 and VeriBlot for IP Detection Reagent (HRP) (ab131366) at a dilution of 1/10000. Lane 1: Mouse brain whole cell lysate
Lane 2: Mouse brain whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab45152 in Mouse brain whole cell lysate
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (acetyl K5) antibody [EP856Y] - BSA and Azide free (ab247302)

This data was developed using ab45152, the same antibody clone in a different buffer formulation.
ab45152 staining Histone H2A (acetyl K5) in rat liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/2000. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500. Negative control 1: PBS in place of primary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (acetyl K5) antibody [EP856Y] - BSA and Azide free (ab247302)

This data was developed using ab45152, the same antibody clone in a different buffer formulation.
ab45152 staining Histone H2A (acetyl K5) in mouse liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/2000. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500. Negative control 1: PBS in place of primary antibody.
Western blot - Anti-Histone H2A (acetyl K5) antibody [EP856Y] - BSA and Azide free (ab247302)

All lanes : Anti-Histone H2A (acetyl K5) antibody [EP856Y] (ab45152) at 1/100000 dilution (unpurified)

Lane 1 : C6 cell lysate
Lane 2 : C6 + TSA membrane

Predicted band size: 14 kDa
Observed band size: 14 kDa
Additional bands at: 34 kDa. We are unsure as to the identity of these extra bands.

This data was developed using ab45152, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (acetyl K5) antibody [EP856Y] - BSA and Azide free (ab247302)

This data was developed using ab45152, the same antibody clone in a different buffer formulation.ab45152 staining Histone H2A (acetyl K5) in human colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/2000. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500. Negative control 1: PBS in place of primary antibody.
Anti-Histone H2A (acetyl K5) antibody [EP856Y] - BSA and Azide free (ab247302)

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