Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Histone H2A (acetyl K5) antibody [EP856Y] - BSA and Azide free (ab247302)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP856Y] to Histone H2A (acetyl K5) - BSA and Azide free
  • Suitable for: IP, ICC/IF, IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Histone H2A (acetyl K5) antibody [EP856Y] - BSA and Azide free
    See all Histone H2A primary antibodies

  • Description

    Rabbit monoclonal [EP856Y] to Histone H2A (acetyl K5) - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, ICC/IF, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Caenorhabditis elegans

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab247302 is the carrier-free version of ab45152. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab247302 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EP856Y

  • Isotype

    IgG

Images

  • Immunocytochemistry - Anti-Histone H2A (acetyl K5) antibody [EP856Y] - BSA and Azide free (ab247302)
    Immunocytochemistry - Anti-Histone H2A (acetyl K5) antibody [EP856Y] - BSA and Azide free (ab247302)

    This data was developed using ab45152, the same antibody clone in a different buffer formulation.

    ab45152 staining Histone H2A (acetyl K5) in C6 (rat glioma) cells, treated and untreated with TSA,500ng/ml for 4 hours by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/200. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody. ab7291 and ab150120 were used as counterstains for primary antibody ab75748 and secondary antibody ab150077 respectively and DAPI was used as a nuclear counterstain. Negative control 1: Rabbit primary antibody and anti-mouse secondary antibody (ab150120)
    Negative control 2: Mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab150077)
  • Immunoprecipitation - Anti-Histone H2A (acetyl K5) antibody [EP856Y] - BSA and Azide free (ab247302)
    Immunoprecipitation - Anti-Histone H2A (acetyl K5) antibody [EP856Y] - BSA and Azide free (ab247302)

    This data was developed using ab45152, the same antibody clone in a different buffer formulation.

    ab45152 immunoprecipitating Histone H2A. 10µg of cell lysate was incubated with primary antibody at a dilution of 1/20 and VeriBlot for IP Detection Reagent (HRP) (ab131366) at a dilution of 1/10000. Lane 1: Mouse brain whole cell lysate
    Lane 2: Mouse brain whole cell lysate
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab45152 in Mouse brain whole cell lysate
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (acetyl K5) antibody [EP856Y] - BSA and Azide free (ab247302)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (acetyl K5) antibody [EP856Y] - BSA and Azide free (ab247302)

    This data was developed using ab45152, the same antibody clone in a different buffer formulation.

    ab45152 staining Histone H2A (acetyl K5) in rat liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/2000. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500. Negative control 1: PBS in place of primary antibody.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (acetyl K5) antibody [EP856Y] - BSA and Azide free (ab247302)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (acetyl K5) antibody [EP856Y] - BSA and Azide free (ab247302)

    This data was developed using ab45152, the same antibody clone in a different buffer formulation.

    ab45152 staining Histone H2A (acetyl K5) in mouse liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/2000. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500. Negative control 1: PBS in place of primary antibody.
  • Western blot - Anti-Histone H2A (acetyl K5) antibody [EP856Y] - BSA and Azide free (ab247302)
    Western blot - Anti-Histone H2A (acetyl K5) antibody [EP856Y] - BSA and Azide free (ab247302)
    All lanes : Anti-Histone H2A (acetyl K5) antibody [EP856Y] (ab45152) at 1/100000 dilution (unpurified)

    Lane 1 : C6 cell lysate
    Lane 2 : C6 + TSA membrane

    Predicted band size: 14 kDa
    Observed band size: 14 kDa
    Additional bands at: 34 kDa. We are unsure as to the identity of these extra bands.



    This data was developed using ab45152, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (acetyl K5) antibody [EP856Y] - BSA and Azide free (ab247302)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (acetyl K5) antibody [EP856Y] - BSA and Azide free (ab247302)
    This data was developed using ab45152, the same antibody clone in a different buffer formulation.ab45152 staining Histone H2A (acetyl K5) in human colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/2000. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500. Negative control 1: PBS in place of primary antibody.
  • Anti-Histone H2A (acetyl K5) antibody [EP856Y] - BSA and Azide free (ab247302)
    Anti-Histone H2A (acetyl K5) antibody [EP856Y] - BSA and Azide free (ab247302)

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