Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PRX antibody [EPR24150-36] - BSA and Azide free (ab278083)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR24150-36] to PRX - BSA and Azide free
  • Suitable for: IHC-Fr, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-PRX antibody [EPR24150-36] - BSA and Azide free
    See all PRX primary antibodies

  • Description

    Rabbit monoclonal [EPR24150-36] to PRX - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-Fr, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF,IP or WB

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human lung, cerebrum and skeletal muscle tissue; Mouse lung, cerebrum and skeletal muscle tissue; Rat lung and skeletal muscle tissue. IHC-Fr: Rat sciatic nerve and lung tissue; Mouse sciatic nerve and lung tissue

  • General notes

    ab278083 is the carrier-free version of ab259953.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRX antibody [EPR24150-36] - BSA and Azide free (ab278083)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRX antibody [EPR24150-36] - BSA and Azide free (ab278083)

    This data was developed using ab259953, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling PRX with ab259953 at 1/2000 (0.24 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on the capillaries of human lung. The section was incubated with ab259953 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND®RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond®Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRX antibody [EPR24150-36] - BSA and Azide free (ab278083)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRX antibody [EPR24150-36] - BSA and Azide free (ab278083)

    This data was developed using ab259953, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling PRX with abab259953 at 1/2000 (0.24 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on the capillaries of mouse lung (PMID: 29968755). The section was incubated with ab259953 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) was used.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Frozen sections) - Anti-PRX antibody [EPR24150-36] - BSA and Azide free (ab278083)
    Immunohistochemistry (Frozen sections) - Anti-PRX antibody [EPR24150-36] - BSA and Azide free (ab278083)

    This data was developed using ab259953, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse lung tissue labeling PRX with ab259953 at 1/100 (4.8 ug/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on mouse lung is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRX antibody [EPR24150-36] - BSA and Azide free (ab278083)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRX antibody [EPR24150-36] - BSA and Azide free (ab278083)

    This data was developed using ab259953, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling PRX with abab259953 at 1/2000 (0.24 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond®Polymer Refine Detection). Positive staining on the capillaries of human cerebrum (PMID: 29968755). The section was incubated with ab259953 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND®RX instrument.  Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) was used.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Frozen sections) - Anti-PRX antibody [EPR24150-36] - BSA and Azide free (ab278083)
    Immunohistochemistry (Frozen sections) - Anti-PRX antibody [EPR24150-36] - BSA and Azide free (ab278083)

    This data was developed using ab259953, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat sciatic nerve tissue labeling PRX with ab259953 at 1/250 (1.92 ug/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on rat sciatic nerve is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRX antibody [EPR24150-36] - BSA and Azide free (ab278083)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRX antibody [EPR24150-36] - BSA and Azide free (ab278083)

    This data was developed using ab259953, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling PRX with abab259953 at 1/2000 (0.24 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on the nerve fibers of human skeletal muscle. The section was incubated with ab259953 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRX antibody [EPR24150-36] - BSA and Azide free (ab278083)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRX antibody [EPR24150-36] - BSA and Azide free (ab278083)

    This data was developed using ab259953, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissue labeling PRX with abab259953 at 1/2000 (0.24 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on the nerve fibers of mouse skeletal muscle. The section was incubated with ab259953 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Frozen sections) - Anti-PRX antibody [EPR24150-36] - BSA and Azide free (ab278083)
    Immunohistochemistry (Frozen sections) - Anti-PRX antibody [EPR24150-36] - BSA and Azide free (ab278083)

    This data was developed using ab259953, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat lung tissue labeling PRX with ab259953 at 1/100 (4.8 ug/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on rat lung is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Immunohistochemistry (Frozen sections) - Anti-PRX antibody [EPR24150-36] - BSA and Azide free (ab278083)
    Immunohistochemistry (Frozen sections) - Anti-PRX antibody [EPR24150-36] - BSA and Azide free (ab278083)

    This data was developed using ab259953, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse sciatic nerve tissue labeling PRX with ab259953 at 1/250 (1.92 ug/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on mouse sciatic nerve is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRX antibody [EPR24150-36] - BSA and Azide free (ab278083)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRX antibody [EPR24150-36] - BSA and Azide free (ab278083)

    This data was developed using ab259953, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Rat lung tissue labeling PRX with abab259953 at 1/2000 (0.24 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on the capillaries of rat lung. The section was incubated with ab259953 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRX antibody [EPR24150-36] - BSA and Azide free (ab278083)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRX antibody [EPR24150-36] - BSA and Azide free (ab278083)

    This data was developed using ab259953, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Rat skeletal muscle tissue labeling PRX with abab259953 at 1/2000 (0.24 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on the nerve fibers of rat skeletal muscle. The section was incubated with ab259953 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) was used.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRX antibody [EPR24150-36] - BSA and Azide free (ab278083)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRX antibody [EPR24150-36] - BSA and Azide free (ab278083)

    This data was developed using ab259953, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling PRX with abab259953 at 1/2000 (0.24 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Negative control: no staining on the capillaries of mouse cerebrum (PMID:29968755). The section was incubated with ab259953 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Anti-PRX antibody [EPR24150-36] - BSA and Azide free (ab278083)
    Anti-PRX antibody [EPR24150-36] - BSA and Azide free (ab278083)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

© 2021 Astana Biomed Group LLP. All rights reserved.