Anti-TR4 antibody [EPR1773(2)] - BSA and Azide free (ab247826)
![Anti-TR4 antibody [EPR1773(2)] - BSA and Azide free (ab247826)](https://www.abcam.com/ps/products/247/ab247826/Images/ab247826-445636-anti-tr4-antibody-epr1773-2-bsa-and-azide-free-western-blot.jpg "Anti-TR4 antibody [EPR1773(2)] - BSA and Azide free (ab247826)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1773(2)] to TR4 - BSA and Azide free
- Suitable for: IP, WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-TR4 antibody [EPR1773(2)] - BSA and Azide free
See all TR4 primary antibodies -
Description
Rabbit monoclonal [EPR1773(2)] to TR4 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details
Unsuitable for: ICC/IF or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab247826 is the carrier-free version of ab109301.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR1773(2) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-TR4 antibody [EPR1773(2)] - BSA and Azide free (ab247826)All lanes : Anti-TR4 antibody [EPR1773(2)] (ab109301) at 1/5000 dilution (purified)
Lane 1 : PC-12 whole cell lysate
Lane 2 : 3T3-L1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 65 kDa
Observed band size: 67 kDa why is the actual band size different from the predicted?This data was developed using ab109301, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-TR4 antibody [EPR1773(2)] - BSA and Azide free (ab247826)](https://www.abcam.com/ps/products/247/ab247826/Images/ab247826-410658-anti-tr4-antibody-epr1773-2-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-TR4 antibody [EPR1773(2)] - BSA and Azide free (ab247826)Anti-TR4 antibody [EPR1773(2)] (ab109301) at 1/5000 dilution (purified) + HEK-293 whole cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 65 kDa
Observed band size: 67 kDa why is the actual band size different from the predicted?This data was developed using ab109301, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-TR4 antibody [EPR1773(2)] - BSA and Azide free (ab247826)](https://www.abcam.com/ps/products/247/ab247826/Images/ab247826-408066-anti-tr4-antibody-epr1773-2-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-TR4 antibody [EPR1773(2)] - BSA and Azide free (ab247826)Anti-TR4 antibody [EPR1773(2)] (ab109301) at 1/2000 dilution (purified) + PC-3 whole cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 65 kDa
Observed band size: 67 kDa why is the actual band size different from the predicted?This data was developed using ab109301, the same antibody clone in a different buffer formulation.
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![Immunoprecipitation - Anti-TR4 antibody [EPR1773(2)] - BSA and Azide free (ab247826)](https://www.abcam.com/ps/products/247/ab247826/Images/ab247826-3-anti-tr4-antibody-epr1773-2-bsa-and-azide-free-immunoprecipitation.jpg)
Immunoprecipitation - Anti-TR4 antibody [EPR1773(2)] - BSA and Azide free (ab247826)This data was developed using ab109301, the same antibody clone in a different buffer formulation.ab109301 (purified) at 1/30 immunoprecipitating TR4 in 10 μg HEK293 cell lysate (Lanes 1 and 2, observed at 67 kDa). Lane 3 - Rabbit monoclonal IgG (ab172730). For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution. Blocking buffer and concentration: 5% NFDM/TBST Dilution buffer and concentration: 5% NFDM/TBST -
![Western blot - Anti-TR4 antibody [EPR1773(2)] - BSA and Azide free (ab247826)](https://www.abcam.com/ps/products/247/ab247826/Images/ab247826-402800-anti-tr4-antibody-epr1773-2-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-TR4 antibody [EPR1773(2)] - BSA and Azide free (ab247826)This data was developed using ab109301, the same antibody clone in a different buffer formulation.
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![Western blot - Anti-TR4 antibody [EPR1773(2)] - BSA and Azide free (ab247826)](https://www.abcam.com/ps/products/247/ab247826/Images/ab247826-397746-anti-tr4-antibody-epr17732-western-blot-human-knockout-lysate.jpg)
Western blot - Anti-TR4 antibody [EPR1773(2)] - BSA and Azide free (ab247826)All lanes : Anti-TR4 antibody [EPR1773(2)] (ab109301) at 1/1000 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : NR2C2 knockout HEK293T cell lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 65 kDa
Observed band size: 67 kDa why is the actual band size different from the predicted?This data was developed using ab109301, the same antibody clone in a different buffer formulation.
Lanes 1-3: Merged signal (red and green). Green - ab109301 observed at 67 kDa. Red - loading control ab8245 observed at 36 kDa.
ab109301 Anti-TR4 antibody [EPR1773(2)] was shown to specifically react with TR4 in wild-type HEK293T cells. The band observed in knockout cell line ab266228 (knockout cell lysate ab257563) lane below 67 kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type and TR4 knockout samples were subjected to SDS-PAGE. ab109301 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Anti-TR4 antibody [EPR1773(2)] - BSA and Azide free (ab247826)](https://www.abcam.com/ps/products/247/ab247826/Images/ab247826-6-benefits-of-recombinant-antibodies.png)
Anti-TR4 antibody [EPR1773(2)] - BSA and Azide free (ab247826)