Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PP-X antibody [EPR19502] - BSA and Azide free (ab251204)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19502] to PP-X - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-PP-X antibody [EPR19502] - BSA and Azide free
    See all PP-X primary antibodies

  • Description

    Rabbit monoclonal [EPR19502] to PP-X - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab251204 is the carrier-free version of ab195371.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-PP-X antibody [EPR19502] - BSA and Azide free (ab251204)
    Western blot - Anti-PP-X antibody [EPR19502] - BSA and Azide free (ab251204)
    Lanes 1-2 : Anti-PP-X antibody [EPR19502] (ab195371) at 1/1000 dilution
    Lane 3 : Anti-PP-X antibody [EPR19502] (ab195371) at 1/10000 dilution

    Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
    Lane 3 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 35 kDa
    Observed band size: 35 kDa


    Exposure time: 30 seconds


    This data was developed using ab195371, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-PP-X antibody [EPR19502] - BSA and Azide free (ab251204)
    Western blot - Anti-PP-X antibody [EPR19502] - BSA and Azide free (ab251204)
    All lanes : Anti-PP-X antibody [EPR19502] (ab195371) at 1/1000 dilution

    Lane 1 : Human liver tissue lysate
    Lane 2 : Human kidney tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 35 kDa
    Observed band size: 35 kDa


    Exposure time: 3 minutes


    This data was developed using ab195371, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-PP-X antibody [EPR19502] - BSA and Azide free (ab251204)
    Western blot - Anti-PP-X antibody [EPR19502] - BSA and Azide free (ab251204)
    All lanes : Anti-PP-X antibody [EPR19502] (ab195371) at 1/1000 dilution

    Lane 1 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 2 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
    Lane 3 : Mouse kidney tissue lysate
    Lane 4 : Mouse spleen tissue lysate
    Lane 5 : Rat brain tissue lysate
    Lane 6 : Rat kidney tissue lysate
    Lane 7 : Rat spleen tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 35 kDa
    Observed band size: 35 kDa



    This data was developed using ab195371, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PP-X antibody [EPR19502] - BSA and Azide free (ab251204)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PP-X antibody [EPR19502] - BSA and Azide free (ab251204)
    This data was developed using ab195371, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labeling PP-X with ab195371 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Strong nuclear and weak cytoplasmic staining on human colon carcinoma is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PP-X antibody [EPR19502] - BSA and Azide free (ab251204)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PP-X antibody [EPR19502] - BSA and Azide free (ab251204)
    This data was developed using ab195371, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human pancreatic carcinoma tissue labeling PP-X with ab195371 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic and nuclear staining on human pancreatic carcinoma is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Flow Cytometry - Anti-PP-X antibody [EPR19502] - BSA and Azide free (ab251204)
    Flow Cytometry - Anti-PP-X antibody [EPR19502] - BSA and Azide free (ab251204)
    This data was developed using ab195371, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 4% paraformaldehyde-fixed NIH/3T3 (Mouse embryonic fibroblast cell line) cells labeling PP-X with ab195371 at 1/500 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody; blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.
  • Anti-PP-X antibody [EPR19502] - BSA and Azide free (ab251204)
    Anti-PP-X antibody [EPR19502] - BSA and Azide free (ab251204)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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