Anti-NIPP1 antibody (ab5300)
Key features and details
- Goat polyclonal to NIPP1
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-NIPP1 antibody
See all NIPP1 primary antibodies -
Description
Goat polyclonal to NIPP1 -
Host species
Goat -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB MouseHuman
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Immunogen
Synthetic peptide corresponding to Human NIPP1 aa 114-127 (C terminal).
Sequence:C-EAWPGKKPTPSLLI
(Peptide available asab23096) -
Positive control
- WB: HeLa, HepG2, Jurkat and NIH3T3 nuclear lysates. IHC-P: Human thymus tissue.
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General notes
This gene, through alternative splicing, encodes three different isoforms. Two of the protein isoforms encoded by this gene are specific inhibitors of type 1 serine/threonine protein phosphatases and can bind but not cleave RNA. The third protein isoform lacks the phosphatase inhibitory function but is a single-strand endoribonuclease comparable to RNase E of E. coli. This isoform requires magnesium for its function and cleaves specific sites in A+U-rich regions of RNA.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Primary antibody notes
This gene, through alternative splicing, encodes three different isoforms. Two of the protein isoforms encoded by this gene are specific inhibitors of type 1 serine/threonine protein phosphatases and can bind but not cleave RNA. The third protein isoform lacks the phosphatase inhibitory function but is a single-strand endoribonuclease comparable to RNase E of E. coli. This isoform requires magnesium for its function and cleaves specific sites in A+U-rich regions of RNA. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-NIPP1 antibody (ab5300)All lanes : Anti-NIPP1 antibody (ab5300) at 1 µg/ml
Lane 1 : HeLa nuclear lysate in RIPA buffer
Lane 2 : HepG2 nuclear lysate in RIPA buffer
Lane 3 : Jurkat nuclear lysate in RIPA buffer
Lane 4 : NIH3T3 nuclear lysate in RIPA buffer
Lysates/proteins at 35 µg per lane.
Predicted band size: 40 kDaPrimary incubation for 1 hour. Detected using chemiluminescence.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NIPP1 antibody (ab5300)ab5300 (3.8µg/ml) staining of paraffin embedded Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
