Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PPP2R1B antibody [EPR10158] (ab154815)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10158] to PPP2R1B
  • Suitable for: WB, Flow Cyt, ICC/IF
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-PPP2R1B antibody [EPR10158]
    See all PPP2R1B primary antibodies

  • Description

    Rabbit monoclonal [EPR10158] to PPP2R1B

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human PPP2R1B (internal sequence). The exact sequence is proprietary.

  • Positive control

    • WB: HEK293T, HeLa, HAP1, Caco 2 and Jurkat whole cell lysate (ab7899). ICC/IF: Caco-2 cells. Flow Cyt: Caco-2 cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20ºC.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR10158

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-PPP2R1B antibody [EPR10158] (ab154815)
    Western blot - Anti-PPP2R1B antibody [EPR10158] (ab154815)
    All lanes : Anti-PPP2R1B antibody [EPR10158] (ab154815) at 1/1000 dilution

    Lane 1 : Wild-type HEK293T cell lysate
    Lane 2 : PPP2R1B knockout HEK293T cell lysate
    Lane 3 : Caco-2 cell lysate
    Lane 4 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 66 kDa



    Lanes 1-4: Merged signal (red and green). Green - ab154815 observed at 66 kDa. Red - loading control ab8245 observed at 36 kDa.

     ab154815 Anti-PPP2R1B antibody [EPR10158]  was shown to specifically react with PPP2R1B in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266729 (knockout cell lysate ab258133) was used. Wild-type and PPP2R1B knockout samples were subjected to SDS-PAGE. ab154815 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Flow Cytometry - Anti-PPP2R1B antibody [EPR10158] (ab154815)
    Flow Cytometry - Anti-PPP2R1B antibody [EPR10158] (ab154815)

    Flow Cytometry analysis of Caco-2 (human colorectal adenocarcinoma ) cells labeling PPP2R1B with unpurified ab154815 at 1:40 dilution(10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(ab150077)(1:2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black)(ab172730) was used as the isotype control, Cell without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

  • Western blot - Anti-PPP2R1B antibody [EPR10158] (ab154815)
    Western blot - Anti-PPP2R1B antibody [EPR10158] (ab154815)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: PPP2R1B knockout HAP1 cell lysate (20 µg)
    Lane 3: HeLa cell lysate (20 µg)
    Lane 4: Caco2 cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab154815 observed at 67 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab154815 was shown to specifically react with PPP2R1B when PPP2R1B knockout samples were used. Wild-type and PPP2R1B knockout samples were subjected to SDS-PAGE. ab154815 and ab8245 (loading control to GAPDH) were diluted at 1/1000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-PPP2R1B antibody [EPR10158] (ab154815)
    Western blot - Anti-PPP2R1B antibody [EPR10158] (ab154815)
    All lanes : Anti-PPP2R1B antibody [EPR10158] (ab154815) at 1/1000 dilution

    Lane 1 : Mouse spleen tissue lysate
    Lane 2 : Rat spleen tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG) at 1/2000 dilution

    Predicted band size: 66 kDa
    Observed band size: 66 kDa


    Exposure time: 3 minutes
  • Western blot - Anti-PPP2R1B antibody [EPR10158] (ab154815)
    Western blot - Anti-PPP2R1B antibody [EPR10158] (ab154815)
    All lanes : Anti-PPP2R1B antibody [EPR10158] (ab154815) at 1/1000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : Caco 2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 66 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-PPP2R1B antibody [EPR10158] (ab154815)
    Immunocytochemistry/ Immunofluorescence - Anti-PPP2R1B antibody [EPR10158] (ab154815)
    Immunofluorescence analysis of Caco 2 cells, labeling PPP2R1B using ab154815 at 1/50 dilution.
  • Anti-PPP2R1B antibody [EPR10158] (ab154815)
    Anti-PPP2R1B antibody [EPR10158] (ab154815)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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