Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PPP2R1B antibody [EPR10159] (ab154833)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10159] to PPP2R1B
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-PPP2R1B antibody [EPR10159]
    See all PPP2R1B primary antibodies

  • Description

    Rabbit monoclonal [EPR10159] to PPP2R1B

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to Human PPP2R1B.
    Database link: P30154

  • Positive control

    • Caco2 and Jurkat cell lysates.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20ºC.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR10159

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-PPP2R1B antibody [EPR10159] (ab154833)
    Western blot - Anti-PPP2R1B antibody [EPR10159] (ab154833)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: PPP2R1B knockout HAP1 cell lysate (20 µg)
    Lane 3: Jurkat cell lysate (20 µg)
    Lane 4: Caco2 cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab154833 observed at 67 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab154833 was shown to recognize PPP2R1B when PPP2R1B knockout samples were used, along with additional cross-reactive bands. Wild-type and PPP2R1B knockout samples were subjected to SDS-PAGE. ab154833 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-PPP2R1B antibody [EPR10159] (ab154833)
    Western blot - Anti-PPP2R1B antibody [EPR10159] (ab154833)
    All lanes : Anti-PPP2R1B antibody [EPR10159] (ab154833) at 1/1000 dilution

    Lane 1 : Caco2 cell lysate
    Lane 2 : Jurkat cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 66 kDa

  • Anti-PPP2R1B antibody [EPR10159] (ab154833)
    Anti-PPP2R1B antibody [EPR10159] (ab154833)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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