Anti-Syntaxin 6 antibody [3D10] - Golgi Membrane Marker (ab12370)
![Anti-Syntaxin 6 antibody [3D10] - Golgi Membrane Marker (ab12370)](https://www.abcam.com/ps/products/12/ab12370/Images/ab12370-20035-anti-syntaxin-6-antibody-3d10-western-blot.jpg "Anti-Syntaxin 6 antibody [3D10] - Golgi Membrane Marker (ab12370)")
Key features and details
- Mouse monoclonal [3D10] to Syntaxin 6 - Golgi Membrane Marker
- Suitable for: Flow Cyt, WB, IP
- Reacts with: Mouse, Rat
- Isotype: IgG1
Overview
-
Product name
Anti-Syntaxin 6 antibody [3D10] - Golgi Membrane Marker
See all Syntaxin 6 primary antibodies -
Description
Mouse monoclonal [3D10] to Syntaxin 6 - Golgi Membrane Marker -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt RatIP MouseWB MouseRat
-
Immunogen
Recombinant full length protein corresponding to Rat Syntaxin 6.
-
Epitope
The epitope for this antibody has been localized to the amino terminus (residues 1-25) of Syntaxin 6. -
Positive control
- Mouse Brain Tissue Extract, rat Brain Tissue Extract.
-
General notes
This product was changed from ascites to tissue culture supernatant on 5th July 2019. Lot numbers higher than GR3173630 are from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from TCS -
Clonality
Monoclonal -
Clone number
3D10 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
-
Western blot - Anti-Syntaxin 6 antibody [3D10] - Golgi Membrane Marker (ab12370)All lanes : Anti-Syntaxin 6 antibody [3D10] - Golgi Membrane Marker (ab12370) at 1 µg/ml
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Brain (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 30.6 kDa
Observed band size: 30.6 kDa
Additional bands at: 26 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutesThis image was generated using the ascites version of the product.
-
![Western blot - Anti-Syntaxin 6 antibody [3D10] - Golgi Membrane Marker (ab12370)](https://www.abcam.com/ps/products/12/ab12370/Images/ab12370-20034-anti-syntaxin-6-antibody-3d10-western-blot.jpg)
Western blot - Anti-Syntaxin 6 antibody [3D10] - Golgi Membrane Marker (ab12370) Image from Jonas MC et al, J Cell Sci. 2010 Oct 1;123(Pt 19):3378-88. Epub 2010 Sep 7, Fig 1, doi:10.1242/jcs.068841.Intracellular membranes from CHO cells were separated on a 10–24% discontinuous Nycodenz gradient. Western Blotting was then performed using a range of antibodies including ab12370 at a 1/1000 dilution. An HRP-conjugated anti-mouse secondary was used at a 1/6000 dilution.
This image was generated using the ascites version of the product.
-
![Immunoprecipitation - Anti-Syntaxin 6 antibody [3D10] - Golgi Membrane Marker (ab12370)](https://www.abcam.com/ps/products/12/ab12370/Images/ab12370-20033-anti-syntaxin-6-antibody-3d10-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Syntaxin 6 antibody [3D10] - Golgi Membrane Marker (ab12370)Syntaxin 6 was immunoprecipitated using 0.5mg Mouse Brain whole tissue lysate, 10µg of Mouse monoclonal to Syntaxin 6 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Mouse Brain whole tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab12370.
Secondary: Protein G-HRP at 1/500 dilution.
Band: 25kDa:Syntaxin 6.This image was generated using the ascites version of the product.
-
![Flow Cytometry - Anti-Syntaxin 6 antibody [3D10] - Golgi Membrane Marker (ab12370)](https://www.abcam.com/ps/products/12/ab12370/Images/ab12370-20032-anti-syntaxin-6-antibody-3d10-flow-cytometry.jpg)
Flow Cytometry - Anti-Syntaxin 6 antibody [3D10] - Golgi Membrane Marker (ab12370)Overlay histogram showing PC12 cells stained with ab12370 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab12370, 2µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1](ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
This image was generated using the ascites version of the product.
