Anti-TOM1L2 antibody (ab96320)
Key features and details
- Rabbit polyclonal to TOM1L2
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TOM1L2 antibody
See all TOM1L2 primary antibodies -
Description
Rabbit polyclonal to TOM1L2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide corresponding to a region within the internal sequence amino acids 394-457 (
SLAEQRKTVTYEDPQAVGGLASALDNRKQSSEGIPVAQPSVMDDIEVWLR TDLKGDDLEEGVTS
) of Human TOM1L2. -
Positive control
- A549, HeLa, Jurkat and NT2D1 cells
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-TOM1L2 antibody (ab96320)
TOM1L2 was immunoprecipitated using 0.5mg Jurkat whole cell extract, 5µg of Rabbit polyclonal to TOM1L2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Jurkat whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab96320.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697)
>Band: 56kDa; TOM1L2
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Western blot - Anti-TOM1L2 antibody (ab96320)All lanes : Anti-TOM1L2 antibody (ab96320) at 1/1000 dilution
Lane 1 : Jurkat whole cell lysate
Lane 2 : NT2D1 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 56 kDa
7.5% SDS PAGE
