Anti-Perilipin 3/TIP47 antibody (ab47638)
Key features and details
- Rabbit polyclonal to Perilipin 3/TIP47
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Perilipin 3/TIP47 antibody
See all Perilipin 3/TIP47 primary antibodies -
Description
Rabbit polyclonal to Perilipin 3/TIP47 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Daudi cell lysate, Rat Liver tissue lysate; IHC-P: Rat Liver tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Perilipin 3/TIP47 antibody (ab47638)Lane 1 : Anti-Perilipin 3/TIP47 antibody (ab47638) at 1 µg/ml
Lane 2 : Anti-Perilipin 3/TIP47 antibody (ab47638) at 2 µg/ml
All lanes : Daudi (human Burkitt's lymphoma cell line) cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-rabbit IgG (HRP) at 1/10000 dilution
Predicted band size: 47 kDa
Observed band size: 47 kDaDiluting buffer and concentration: 5% NFDM/TBST.
Primary incubated for 1 hour at room temperature.
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Immunocytochemistry/ Immunofluorescence - Anti-Perilipin 3/TIP47 antibody (ab47638) This image is courtesy of an Abreview submitted by Dimitra Kalamidaab47638 staining Perilipin 3/TIP47 in Human HUVEC cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, pH 7.4 for 5 minutes at room temperature and blocked with 5% BSA for 20 minutes at room temperature. Samples were incubated with primary antibody (1/500 in PBS) for 1 hour. A CF488A-conjugated Donkey anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.
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Western blot - Anti-Perilipin 3/TIP47 antibody (ab47638)Lane 1 : Anti-Perilipin 3/TIP47 antibody (ab47638) at 0.5 µg/ml
Lane 2 : Anti-Perilipin 3/TIP47 antibody (ab47638) at 1 µg/ml
Lane 3 : Anti-Perilipin 3/TIP47 antibody (ab47638) at 2 µg/ml
All lanes : Rat Liver tissue lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-rabbit IgG (HRP) at 1/10000 dilution
Predicted band size: 47 kDa
Observed band size: 47 kDaDiluting buffer and concentration: 5% NFDM/TBST.
Primary incubated for 1 hour at room temperature.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Perilipin 3/TIP47 antibody (ab47638)ab47638 at 10 µg/ml staining Perilipin 3/TIP47 in rat liver tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections).
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Immunocytochemistry/ Immunofluorescence - Anti-Perilipin 3/TIP47 antibody (ab47638)Immunofluorescence of Perilipin 3/TIP47 in Rat Liver cells using ab47638 at 20 ug/ml.
