Anti-ILKAP antibody [EPR16145] - BSA and Azide free (ab251214)
![Anti-ILKAP antibody [EPR16145] - BSA and Azide free (ab251214)](https://www.abcam.com/ps/products/251/ab251214/Images/ab251214-395393-anti-ilkap-antibody-epr16145-western-blot-human-knockout-lysate.jpg "Anti-ILKAP antibody [EPR16145] - BSA and Azide free (ab251214)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16145] to ILKAP - BSA and Azide free
- Suitable for: WB, IP
- Knockout validated
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-ILKAP antibody [EPR16145] - BSA and Azide free
See all ILKAP primary antibodies -
Description
Rabbit monoclonal [EPR16145] to ILKAP - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HEK239T, HeLa and HAP1 cell lysates.
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General notes
Ab251214 is the carrier-free version of ab196013. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab251214 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
EPR16145 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ILKAP antibody [EPR16145] - BSA and Azide free (ab251214)All lanes : Anti-ILKAP antibody [EPR16145] (ab196013) at 1/1000 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : ILKAP knockout HEK293T cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : HAP1 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 43 kDa
Observed band size: 49 kDa why is the actual band size different from the predicted?This data was developed using the same antibody clone in a different buffer formulation (ab196013).
Lanes 1-4: Merged signal (red and green). Green - ab196013 observed at 49 kDa. Red - loading control ab8245 observed at 36 kDa.
ab196013 Anti-ILKAP antibody [EPR16145] was shown to specifically react with ILKAP in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266475 (knockout cell lysate ab258000) was used. Wild-type and ILKAP knockout samples were subjected to SDS-PAGE. ab196013 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Anti-ILKAP antibody [EPR16145] - BSA and Azide free (ab251214)](https://www.abcam.com/ps/products/251/ab251214/Images/ab251214-2-benefits-of-recombinant-antibodies.png)
Anti-ILKAP antibody [EPR16145] - BSA and Azide free (ab251214)
