Human NR2C2 (TR4) knockout HEK-293T cell lysate (ab257563)
Properties
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Storage instructions
Store at -80°C. Please refer to protocols. -
Components 1 kit ab263568 - Human NR2C2 knockout HEK293T cell lysate 1 x 100µg ab255553 - Human wild-type HEK293T cell lysate 1 x 100µg -
Research areas
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Cell type
epithelial -
STR Analysis
Amelogenin X D5S818: 8, 9 D13S317: 12, 14 D7S820: 11 D16S539: 9, 13 vWA: 16, 19 TH01: 7, 9.3 TPOX: 11 CSF1PO: 11, 12
Images
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Western blot - Human NR2C2 (TR4) knockout HEK293T cell lysate (ab257563)
Lane 1:Wild-type HEK293T cell lysate (20 ug)
Lane 2:NR2C2 knockout HEK293T cell lysate (20 ug)
Lane 3:HeLa cell lysate (20 ug)ab109301 Anti-TR4 antibody [EPR1773(2)] was shown to specifically react with TR4 in wild-type HEK293T cells. The band observed in knockout cell line ab266228 (knockout cell lysate ab257563) lane below 67 kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type and TR4 knockout samples were subjected to SDS-PAGE. ab109301 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Sanger Sequencing - Human NR2C2 knockout HEK293T cell lysate (ab257563)Allele-1: 16 bp deletion in exon6
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Sanger Sequencing - Human NR2C2 knockout HEK293T cell lysate (ab257563)Allele-2: Insertion of the selection cassette in exon6