Antibodies, Proteins, Kits and Reagents for Life Science

264 681 ₸ Product size

100 µl 264 681 ₸

Order now and get it on Wednesday March 10, 2021

Anti-MRPP3 antibody [EPR14321-30] (ab185942)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR14321-30] to MRPP3
Suitable for: ICC/IF, WB, IP
Reacts with: Human

Product name

Anti-MRPP3 antibody [EPR14321-30]
See all MRPP3 primary antibodies
Description

Rabbit monoclonal [EPR14321-30] to MRPP3
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- K562, 293, HeLa and U87-MG cell lysates; HeLa cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR14321-30
Isotype

IgG
Research areas

Western blot - Anti-MRPP3 antibody [EPR14321-30] (ab185942)

All lanes : Anti-MRPP3 antibody [EPR14321-30] (ab185942) at 1/5000 dilution

Lane 1 : K562 cell lysate
Lane 2 : 293 cell lysate
Lane 3 : HeLa cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 67 kDa
Western blot - Anti-MRPP3 antibody [EPR14321-30] (ab185942)

Anti-MRPP3 antibody [EPR14321-30] (ab185942) at 1/1000 dilution + U87-MG cell lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 67 kDa
Immunocytochemistry/ Immunofluorescence - Anti-MRPP3 antibody [EPR14321-30] (ab185942)

Immunofluorescent analysis of acetone-fixed HeLa cells labeling MRPP3 with ab185942 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor®555) secondary antibody at 1/200 dilution. Counter stained with Dapi.
Immunoprecipitation - Anti-MRPP3 antibody [EPR14321-30] (ab185942)

Western blot analysis of MRPP3 in 293 cell lysate immunoprecipitated using ab185942 at 1/50 dilution (Lane 1). Lane 2: PBS instead of 293 lysate. Secondary antibody: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.
Anti-MRPP3 antibody [EPR14321-30] (ab185942)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com