Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MRPP3 antibody [EPR14321-30] (ab185942)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14321-30] to MRPP3
  • Suitable for: ICC/IF, WB, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-MRPP3 antibody [EPR14321-30]
    See all MRPP3 primary antibodies

  • Description

    Rabbit monoclonal [EPR14321-30] to MRPP3

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • K562, 293, HeLa and U87-MG cell lysates; HeLa cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-MRPP3 antibody [EPR14321-30] (ab185942)
    Western blot - Anti-MRPP3 antibody [EPR14321-30] (ab185942)
    All lanes : Anti-MRPP3 antibody [EPR14321-30] (ab185942) at 1/5000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : 293 cell lysate
    Lane 3 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 67 kDa

  • Western blot - Anti-MRPP3 antibody [EPR14321-30] (ab185942)
    Western blot - Anti-MRPP3 antibody [EPR14321-30] (ab185942)
    Anti-MRPP3 antibody [EPR14321-30] (ab185942) at 1/1000 dilution + U87-MG cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 67 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-MRPP3 antibody [EPR14321-30] (ab185942)
    Immunocytochemistry/ Immunofluorescence - Anti-MRPP3 antibody [EPR14321-30] (ab185942)

    Immunofluorescent analysis of acetone-fixed HeLa cells labeling MRPP3 with ab185942 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor®555) secondary antibody at 1/200 dilution. Counter stained with Dapi.

  • Immunoprecipitation - Anti-MRPP3 antibody [EPR14321-30] (ab185942)
    Immunoprecipitation - Anti-MRPP3 antibody [EPR14321-30] (ab185942)

    Western blot analysis of MRPP3 in 293 cell lysate immunoprecipitated using ab185942 at 1/50 dilution (Lane 1).  Lane 2: PBS instead of 293 lysate.  Secondary antibody: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.  

  • Anti-MRPP3 antibody [EPR14321-30] (ab185942)
    Anti-MRPP3 antibody [EPR14321-30] (ab185942)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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