Call: +7 771 977 66 65, +7 705 421 2277
Sign in or Register
My basket

Astana Biomed Group, an authorized Abcam distributor in Central Asia

Abiomed homepage

  • Categories
    Signal Transduction
    Cancer
    Epigenetics and Nuclear Signaling
    Immunology
    Cell Biology
    Cardiovascular
    Neuroscience
    Tags & Cell Markers
    Kits/ Lysates/ Other
    Developmental Biology
    Microbiology
    Biochemicals
    Secondary antibodies
    Isotype/Loading Controls
    Antibody Arrays
  • About us
  • Partners
  • Contact
    Address

    Saryarka 32, 18, 010000, Astana city, Kazakhstan

    Telephone +7 771 977 66 65, +7 705 421 2277

    Email

    laboratory@ctlab.kz, orders@abiomed.kz

Back to category

Alexa Fluor® 488 Anti-Nup153 antibody [QE5] (ab204250)

Alexa Fluor® 488 Anti-Nup153 antibody [QE5] (ab204250)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Alexa Fluor® 488 Mouse monoclonal [QE5] to Nup153
  • Suitable for: ICC/IF
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
  • Isotype: IgG1

You may also be interested in

Product image
Human APPL1 (APPL) knockout HeLa cell lysate (ab257836)
Product image
Anti-mouse IgG Fc antibody [RMG06] (ab197780)
Product image
Anti-Septin 3 antibody (ab224332)
Product image
Anti-TNFRSF14/HVEM antibody - BSA and Azide free (Capture) (ab242642)

Overview

  • Product name

    Alexa Fluor® 488 Anti-Nup153 antibody [QE5]
    See all Nup153 primary antibodies
  • Description

    Alexa Fluor® 488 Mouse monoclonal [QE5] to Nup153
  • Host species

    Mouse
  • Conjugation

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    See all applications and species data
  • Immunogen

    Full length protein corresponding to Rat Nup153. (Rat liver nuclear envelope proteins).
    Database link: P49791

  • Positive control

    • ICC/IF: HepG2 cells.
  • General notes

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS
  • Concentration information loading...
  • Purity

    IgG fraction
  • Purification notes

    ab204250 recognises NUP153 as well as two related nuclear pore complex proteins: NUP214 and p62.
  • Clonality

    Monoclonal
  • Clone number

    QE5
  • Isotype

    IgG1
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Nucleus
    • Nuclear Pore
    • Signal Transduction
    • Protein Trafficking
    • Nuclear Import / Export
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • Nuclear Receptors
    • Nuclear Pore Complex

Images

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Nup153 antibody [QE5] (ab204250)
    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Nup153 antibody [QE5] (ab204250)

    ab204250 staining Nup153 in HepG2 cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab204250 at 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    This product also gave a positive signal under the same testing conditions in HepG2 cells fixed with 4% formaldehyde (10 min).

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Alexa Fluor® 488 Anti-Nup153 antibody [QE5] (ab204250)

  •  
  • Product image

    Anti-Nup153 antibody (ab245669)

    Applications: IP, WB

  •  
  • Product image

    Anti-Nup153 antibody [R4C8] (ab81463)

    Applications: ICC/IF, WB

  •  
  • Product image

    Alexa Fluor® 647 Anti-Nup153 antibody [SA1] (ab205845)

    Applications: ICC/IF

  •  
  • Product image

    Anti-Nup153 antibody (ab238150)

    Applications: IHC-P

  •  
  • Product image

    Alexa Fluor® 647 Anti-Nup153 antibody [QE5] (ab204457)

    Applications: ICC/IF

  •  
  • Product image

    Anti-Nup153 antibody [QE5] (ab24700)

    Applications: Flow Cyt, ICC/IF

  •  
  • Product image

    Anti-Nup153 antibody (ab245668)

    Applications: ICC/IF, IP, WB

  •  
  • Product image

    Anti-Nup153 antibody [SA1] (ab96462)

    Applications: ICC/IF, IHC-P

  •  
  • Product image

    Anti-Nup153 antibody [EPR7606(2)(B)] (ab171074)

    Applications: Flow Cyt, ICC/IF, WB

Clear all

Recently viewed products

  •  
  • Product image

    Anti-TPT1L + TCTP antibody [EPR13882(B)] (ab182557)

  •  
  • Product image

    Anti-SA2 antibody [EPR10993] - N-terminal (ab171970)

  •  
  • Product image

    Anti-NAT10 antibody [EPR18663] - BSA and Azide free (ab251186)

  •  
  • Product image

    Anti-GBF1 antibody [EPR14889] - BSA and Azide free (ab250988)

  •  
  • Product image

    Anti-Ku70 antibody [7A9E7] (ab201963)

  •  
  • Product image

    Anti-NDUFB11 antibody [EPR15252] - BSA and Azide free (ab250690)

Get resources and offers direct to your inbox Sign up
© 2021 Astana Biomed Group LLP. All rights reserved.