Anti-Calnexin antibody (ab227310)
Key features and details
- Rabbit polyclonal to Calnexin
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Calnexin antibody
See all Calnexin primary antibodies -
Description
Rabbit polyclonal to Calnexin -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Chicken, Cow, Dog, Pig -
Immunogen
Recombinant fragment within Human Calnexin (internal sequence). The exact sequence is proprietary.
Database link: P27824 -
Positive control
- WB: HEK-293T, PC-12 and Rat2 whole cell extracts; HEK-293T, A431, HeLa and HepG2 whole cell lysate (ab7900); Mouse liver lysate. IHC-P: SW480 xenograft and human breast carcinoma tissues. ICC/IF: HeLa cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Calnexin antibody (ab227310) at 1/10000 dilution
Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lanes 2-3 : Calnexin shRNA transfected HEK-293T whole cell extract
Lysates/proteins at 15 µg per lane.
Predicted band size: 68 kDa7.5% SDS-PAGE gel.
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All lanes : Anti-Calnexin antibody (ab227310) at 1/1000 dilution
Lane 1 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell extract
Lane 2 : Rat2 (rat fibroblast cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 68 kDa7.5% SDS-PAGE gel.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calnexin antibody (ab227310)
Paraffin-embedded SW480 (human) xenograft tissue stained for Calnexin using ab227310 at 1/500 dilution in immunohistochemical analysis.
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Anti-Calnexin antibody (ab227310) at 1/1000 dilution + Mouse liver lysate at 50 µg
Predicted band size: 68 kDa7.5% SDS-PAGE gel.
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All lanes : Anti-Calnexin antibody (ab227310) at 1/10000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 68 kDa7.5% SDS-PAGE gel.
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Ice-cold methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Calnexin (green) using ab227310 at 1/200 dilution in ICC/IF.
Blue: Hoechst 33342 staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calnexin antibody (ab227310)
Paraffin-embedded human breast carcinoma tissue stained for Calnexin using ab227310 at 1/500 dilution in immunohistochemical analysis.