Anti-p53 (phospho S15) antibody [P53S15-1C11] (ab278683)
![Anti-p53 (phospho S15) antibody [P53S15-1C11] (ab278683)](https://www.abcam.com/ps/products/278/ab278683/Images/ab278683-4-p53s151c11c2c12.jpg "Anti-p53 (phospho S15) antibody [P53S15-1C11] (ab278683)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [P53S15-1C11] to p53 (phospho S15)
- Suitable for: Flow Cyt, WB
- Reacts with: Mouse, Human
Overview
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Product name
Anti-p53 (phospho S15) antibody [P53S15-1C11]
See all p53 primary antibodies -
Description
Rabbit monoclonal [P53S15-1C11] to p53 (phospho S15) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt MouseWB Mouse
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Immunogen
Synthetic peptide within Human p53 (phospho S15). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P04637 -
Positive control
- WB: L929 (treated with etoposide) cell extract. Flow cyt: SK-N-MC and C2C12 cells (both treated with staurosporine).
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: 49.88% PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA -
Concentration information loading... -
Purity
Protein A/G purified -
Clonality
Monoclonal -
Clone number
P53S15-1C11 -
Isotype
IgG -
Light chain type
kappa -
Research areas
Images
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Flow Cytometry - Anti-p53 (phospho S15) antibody [P53S15-1C11] (ab278683)
Flow cytometric analysis of C2C12 cells secondary antibody only negative control (blue) or 0.1 µg/mL of isotype control (orange) or untreated (red) or treated with staurosporine (green) using ab278683 at 0.1 µg/mL.
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![Western blot - Anti-p53 (phospho S15) antibody [P53S15-1C11] (ab278683)](https://www.abcam.com/ps/products/278/ab278683/Images/ab278683-453698-anti-p53-western-blot-l929.jpg)
Western blot - Anti-p53 (phospho S15) antibody [P53S15-1C11] (ab278683)All lanes : Anti-p53 (phospho S15) antibody [P53S15-1C11] (ab278683) at 0.05 µg/ml
Lane 1 : L929 cell extract untreated
Lane 2 : L929 cell extract treated with etoposide
Predicted band size: 43 kDa
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![Flow Cytometry - Anti-p53 (phospho S15) antibody [P53S15-1C11] (ab278683)](https://www.abcam.com/ps/products/278/ab278683/Images/ab278683-2-p53s151c11.jpg)
Flow Cytometry - Anti-p53 (phospho S15) antibody [P53S15-1C11] (ab278683)Flow cytometric analysis of SK-N-MC cells secondary antibody only negative control (blue) or untreated (red) or treated with staurosporine (green) using ab278683 at 0.1 µg/mL. -
![Anti-p53 (phospho S15) antibody [P53S15-1C11] (ab278683)](https://www.abcam.com/ps/products/278/ab278683/Images/ab278683-1-benefits-of-recombinant-antibodies.png)
Anti-p53 (phospho S15) antibody [P53S15-1C11] (ab278683)