Antibodies, Proteins, Kits and Reagents for Life Science

Anti-p53 (phospho S15) antibody [P53S15-1C11] (ab278683)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [P53S15-1C11] to p53 (phospho S15)
Suitable for: Flow Cyt, WB
Reacts with: Mouse, Human

Product name

Anti-p53 (phospho S15) antibody [P53S15-1C11]
See all p53 primary antibodies
Description

Rabbit monoclonal [P53S15-1C11] to p53 (phospho S15)
Host species

Rabbit
Tested Applications & Species

Application Species
Flow Cyt
Mouse

WB
Mouse

See all applications and species data
Immunogen

Synthetic peptide within Human p53 (phospho S15). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P04637

Run BLAST with Run BLAST with
Positive control
- WB: L929 (treated with etoposide) cell extract. Flow cyt: SK-N-MC and C2C12 cells (both treated with staurosporine).
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.

Application	Species
Flow Cyt	Mouse
WB	Mouse

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.02% Sodium azide
Constituents: 49.88% PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA
Concentration information loading...
Purity

Protein A/G purified
Clonality

Monoclonal
Clone number

P53S15-1C11
Isotype

IgG
Light chain type

kappa
Research areas

Flow Cytometry - Anti-p53 (phospho S15) antibody [P53S15-1C11] (ab278683)

Flow cytometric analysis of C2C12 cells secondary antibody only negative control (blue) or 0.1 µg/mL of isotype control (orange) or untreated (red) or treated with staurosporine (green) using ab278683 at 0.1 µg/mL.
Western blot - Anti-p53 (phospho S15) antibody [P53S15-1C11] (ab278683)

All lanes : Anti-p53 (phospho S15) antibody [P53S15-1C11] (ab278683) at 0.05 µg/ml

Lane 1 : L929 cell extract untreated
Lane 2 : L929 cell extract treated with etoposide

Predicted band size: 43 kDa
Flow Cytometry - Anti-p53 (phospho S15) antibody [P53S15-1C11] (ab278683)

Flow cytometric analysis of SK-N-MC cells secondary antibody only negative control (blue) or untreated (red) or treated with staurosporine (green) using ab278683 at 0.1 µg/mL.
Anti-p53 (phospho S15) antibody [P53S15-1C11] (ab278683)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com