Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CEBP Alpha/CEBPA antibody [EP708Y] - BSA and Azide free (ab247283)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP708Y] to CEBP Alpha/CEBPA - BSA and Azide free
  • Suitable for: ICC, Flow Cyt, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-CEBP Alpha/CEBPA antibody [EP708Y] - BSA and Azide free
    See all CEBP Alpha/CEBPA primary antibodies

  • Description

    Rabbit monoclonal [EP708Y] to CEBP Alpha/CEBPA - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    This antibody recognizes isoform1 and isoform 4.

  • Tested applications

    Suitable for: ICC, Flow Cyt, WBmore details
    Unsuitable for: IHC or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab247283 is the carrier-free version of ab40761 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab247283 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-CEBP Alpha/CEBPA antibody [EP708Y] - BSA and Azide free (ab247283)
    Western blot - Anti-CEBP Alpha/CEBPA antibody [EP708Y] - BSA and Azide free (ab247283)
    Anti-CEBP Alpha/CEBPA antibody [EP708Y] (ab40761) at 1/1000 dilution + U937 + LPS cell lysate at 10 µg

    Predicted band size: 43 kDa
    Observed band size: 30,43 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab40761, the same antibody clone in a different buffer formulation.

    We are unsure as to the identity of the 30kda band.

  • Immunocytochemistry - Anti-CEBP Alpha/CEBPA antibody [EP708Y] - BSA and Azide free (ab247283)
    Immunocytochemistry - Anti-CEBP Alpha/CEBPA antibody [EP708Y] - BSA and Azide free (ab247283)
    This data was developed using ab40761, the same antibody clone in a different buffer formulation.ab40761 (1/250) staining HeLa cells using immunofluorescence.
  • Flow Cytometry - Anti-CEBP Alpha/CEBPA antibody [EP708Y] - BSA and Azide free (ab247283)
    Flow Cytometry - Anti-CEBP Alpha/CEBPA antibody [EP708Y] - BSA and Azide free (ab247283)
    This data was developed using ab40761, the same antibody clone in a different buffer formulation.Overlay histogram showing HeLa cells stained with ab40761 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab40761, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
  • Anti-CEBP Alpha/CEBPA antibody [EP708Y] - BSA and Azide free (ab247283)
    Anti-CEBP Alpha/CEBPA antibody [EP708Y] - BSA and Azide free (ab247283)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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