Antibodies, Proteins, Kits and Reagents for Life Science

314 937 ₸ Product size

100 µl 314 937 ₸ 10 µl 107 212 ₸

Order now and get it on Tuesday March 09, 2021

Anti-Macrophage Inflammatory Protein 3 alpha antibody [EPR22376-58] (ab224188)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR22376-58] to Macrophage Inflammatory Protein 3 alpha
Suitable for: IHC-P, ICC/IF, Flow Cyt
Reacts with: Human

Product name

Anti-Macrophage Inflammatory Protein 3 alpha antibody [EPR22376-58]
See all Macrophage Inflammatory Protein 3 alpha primary antibodies
Description

Rabbit monoclonal [EPR22376-58] to Macrophage Inflammatory Protein 3 alpha
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- IHC-P: Human tonsil and Human colon tissues. ICC/IF: THP-1 cells. Flow Cyt: THP-1 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR22376-58
Isotype

IgG
Research areas

Immunocytochemistry/ Immunofluorescence - Anti-Macrophage Inflammatory Protein 3 alpha antibody [EPR22376-58] (ab224188)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized THP-1 (human monocytic leukemia monocyte) cells labelling Macrophage Inflammatory Protein 3 alpha with ab224188 at 1/100 dilution, followed by Ab224188 anti-Macrophage Inflammatory Protein 3 alpha ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing increased cytoplasmic staining in THP-1 cells treated with Phorbol-12-myristate-13-acetate (PMA, 100ng/ml) for 56 h, then together with lipopolysaccharide (1ug/ml) and Brefeldin A (300ng/ml) for another 16 h is observed. Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Ab224188 anti-Macrophage Inflammatory Protein 3 alpha ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Macrophage Inflammatory Protein 3 alpha antibody [EPR22376-58] (ab224188)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling Macrophage Inflammatory Protein 3 alpha with ab224188 at 1/2000 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on the epithelial like cells on the human tonsil (PMID: 15307135). Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Flow Cytometry - Anti-Macrophage Inflammatory Protein 3 alpha antibody [EPR22376-58] (ab224188)

Flow cytometric analysis of 4% paraformaldehyde fixed 0.1% Tween-20 permeabilized THP-1 (Human monocytic leukemia monocyte) treated with 100ng/ml PMA for 56h, then together with 1ug/ml lipopolysaccharide and 300ng/ml Brefeldin A for another 16h (Red) / Untreated control (Green) cells labelling Macrophage Inflammatory Protein 3 alpha with ab224188 at 1/600 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730, Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 was used as the secondary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Macrophage Inflammatory Protein 3 alpha antibody [EPR22376-58] (ab224188)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Macrophage Inflammatory Protein 3 alpha with ab224188 at 1/2000 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on the follicle-associated epithelium in the human colon (PMID:14997037). Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Anti-Macrophage Inflammatory Protein 3 alpha antibody [EPR22376-58] (ab224188)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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