Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD68 antibody [KP1] - BSA and Azide free (ab233172)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [KP1] to CD68 - BSA and Azide free
  • Suitable for: Mass Cytometry, ICC/IF, IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-CD68 antibody [KP1] - BSA and Azide free
    See all CD68 primary antibodies

  • Description

    Mouse monoclonal [KP1] to CD68 - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: Mass Cytometry, ICC/IF, IHC-P, WBmore details
    Unsuitable for: Flow Cyt

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Tissue, cells or virus. This information is considered to be commercially sensitive.

  • Positive control

    • WB: Human spleen lysate. IHC-P: Human tonsil and liver tissue. ICC/IF: THP-1 cells. IMC: Human tonsil tissue

  • General notes

    ab233172 is a PBS-only buffer format of ab955.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    KP1

  • Isotype

    IgG1

  • Light chain type

    kappa

  • Research areas

Images

  • Mass Cytometry - Anti-CD68 antibody [KP1] - BSA and Azide free (ab233172)
    Mass Cytometry - Anti-CD68 antibody [KP1] - BSA and Azide free (ab233172) This image is courtesy of the Single Cell & Imaging Mass Cytometry Analysis Platform, Goodman Cancer Research Centre, McGill University

    Imaging Mass Cytometry™ (IMC™) image of human tonsil tissue stained with Anti-CD68 antibody [KP1]. ab233172 (carrier-free antibody, purified) was metal-conjugated using a Maxpar® Antibody Labeling Kit from Fluidigm. Immunostaining was performed according to Fluidigm’s protocols. Briefly, slides were subject to deparaffinization and heat-induced epitope retrieval, followed by overnight incubation at 4°C with an antibody cocktail containing metal-tagged antibodies in blocking buffer. Slides were subsequently washed with 0.2% Triton-X and 1x PBS, counterstained with Cell-ID™ Intercalator-Ir diluted at 1/400 in 1x PBS for 30 min at room temperature, rinsed for 5 min with distilled H2O, and air-dried prior to IMC™ acquisition. IMC™ acquisition was performed using the Fluidigm Hyperion™ Imaging System.

    Imaging Mass Cytometry™, IMC™, Cell-ID™, Hyperion™ and Maxpar® are trademarks of Fluidigm Canada

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [KP1] - BSA and Azide free (ab233172)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [KP1] - BSA and Azide free (ab233172)

    Immunohistochemical analysis of paraffin-embedded human liver tissue labeling CD68 with ab233172 at 1/3000 dilution, followed by Goat Anti-mouse IgG H&L (HRP) ready to use. Cytoplasmic staining on Kupffer cells of human liver (PMID: 12118106) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-mouse IgG H&L (HRP) ready to use.

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [KP1] - BSA and Azide free (ab233172)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [KP1] - BSA and Azide free (ab233172)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CD68 with ab233172 at 1/3000 dilution, followed by Goat Anti-mouse IgG H&L (HRP) ready to use. Cytoplasmic staining on macrophages of human tonsil (PMID: 19543531) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-mouse IgG H&L (HRP) ready to use.

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

     

  • Immunocytochemistry/ Immunofluorescence - Anti-CD68 antibody [KP1] - BSA and Azide free (ab233172)
    Immunocytochemistry/ Immunofluorescence - Anti-CD68 antibody [KP1] - BSA and Azide free (ab233172)

    Immunofluorescent analysis of 100% methanol-fixed THP-1 (human monocytic leukemia cell line) cells labeling CD68 with ab233172 at 1/50 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining in THP-1 cells. The nuclear counter stain is DAPI (blue). Tubulin is detected with Recombinant Anti-beta IV Tubulin antibody [EPR16775] (ab179504) at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) (ab150080) secondary antibody at 1/1000 dilution (red).

    Secondary antibody only control: Used PBS instead of primary antibody, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113) secondary antibody at 1/1000 dilution.

     

  • Western blot - Anti-CD68 antibody [KP1] - BSA and Azide free (ab233172)
    Western blot - Anti-CD68 antibody [KP1] - BSA and Azide free (ab233172)
    Anti-CD68 antibody [KP1] - BSA and Azide free (ab233172) at 1/1000 dilution + Human spleen lysate at 20 µg/ml

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Predicted band size: 37 kDa
    Observed band size: 110 kDa
    why is the actual band size different from the predicted?


    Exposure time: 70 seconds


    Blocking and dilution buffer: 5% NFDM/TBST.

    The observed molecular weight is consistent with the literature (PMID:18405323; PMID:11739566; PMID: 16710801).

  • Anti-CD68 antibody [KP1] - BSA and Azide free (ab233172)
    Anti-CD68 antibody [KP1] - BSA and Azide free (ab233172)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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