Astana Biomed Group, an authorized Abcam distributor in Central Asia

HRP Anti-Fatty Acid Synthase antibody [EPR7466] (ab196854)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • HRP Rabbit monoclonal [EPR7466] to Fatty Acid Synthase
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human
  • Conjugation: HRP

Overview

  • Product name

    HRP Anti-Fatty Acid Synthase antibody [EPR7466]
    See all Fatty Acid Synthase primary antibodies

  • Description

    HRP Rabbit monoclonal [EPR7466] to Fatty Acid Synthase

  • Host species

    Rabbit

  • Conjugation

    HRP

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, HEK293 and A549 whole cell lysates.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Images

  • Western blot - HRP Anti-Fatty Acid Synthase antibody [EPR7466] (ab196854)
    Western blot - HRP Anti-Fatty Acid Synthase antibody [EPR7466] (ab196854)
    All lanes : HRP Anti-Fatty Acid Synthase antibody [EPR7466] (ab196854) at 1/5000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : FASN (Fatty Acid Synthase) knockout HAP1 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 273 kDa
    Observed band size: 260 kDa
    why is the actual band size different from the predicted?


    Exposure time: 1 minute


    ab196854 was shown to specifically react with Fatty Acid Synthase in wild-type HAP1 cells as signal was lost in FASN (Fatty Acid Synthase) knockout cells. Wild-type and FASN (Fatty Acid Synthase) knockout samples were subjected to SDS-PAGE. Ab196854 and ab184095 (Mouse monoclonal [mAbcam 9484] to GAPDH - Loading Control (Alexa Fluor® 680) loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/1000 dilution respectively. The loading control was imaged using the Licor Odyssey CLx prior to blots being developed with ECL technique.

  • Western blot - HRP Anti-Fatty Acid Synthase antibody [EPR7466] (ab196854)
    Western blot - HRP Anti-Fatty Acid Synthase antibody [EPR7466] (ab196854)
    All lanes : HRP Anti-Fatty Acid Synthase antibody [EPR7466] (ab196854) at 1/5000 dilution

    Lane 1 : HeLa whole cell lysate (ab150035)
    Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 3 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 273 kDa
    Observed band size: 273 kDa


    Exposure time: 8 minutes


    This blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab196854 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
  • HRP Anti-Fatty Acid Synthase antibody [EPR7466] (ab196854)
    HRP Anti-Fatty Acid Synthase antibody [EPR7466] (ab196854)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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