HRP Anti-Fatty Acid Synthase antibody [EPR7466] (ab196854)
![HRP Anti-Fatty Acid Synthase antibody [EPR7466] (ab196854)](https://www.abcam.com/ps/products/196/ab196854/Images/ab196854-312028-anti-fatty-acid-synthase-antibody-epr7466-hrp-western-blot.jpg "HRP Anti-Fatty Acid Synthase antibody [EPR7466] (ab196854)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR7466] to Fatty Acid Synthase
- Suitable for: WB
- Knockout validated
- Reacts with: Human
- Conjugation: HRP
Overview
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Product name
HRP Anti-Fatty Acid Synthase antibody [EPR7466]
See all Fatty Acid Synthase primary antibodies -
Description
HRP Rabbit monoclonal [EPR7466] to Fatty Acid Synthase -
Host species
Rabbit -
Conjugation
HRP -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa, HEK293 and A549 whole cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Dissociation constant (KD)
KD = 1.34 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR7466 -
Isotype
IgG -
Research areas
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Images
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Western blot - HRP Anti-Fatty Acid Synthase antibody [EPR7466] (ab196854)All lanes : HRP Anti-Fatty Acid Synthase antibody [EPR7466] (ab196854) at 1/5000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : FASN (Fatty Acid Synthase) knockout HAP1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 273 kDa
Observed band size: 260 kDa why is the actual band size different from the predicted?
Exposure time: 1 minuteab196854 was shown to specifically react with Fatty Acid Synthase in wild-type HAP1 cells as signal was lost in FASN (Fatty Acid Synthase) knockout cells. Wild-type and FASN (Fatty Acid Synthase) knockout samples were subjected to SDS-PAGE. Ab196854 and ab184095 (Mouse monoclonal [mAbcam 9484] to GAPDH - Loading Control (Alexa Fluor® 680) loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/1000 dilution respectively. The loading control was imaged using the Licor Odyssey CLx prior to blots being developed with ECL technique.
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![Western blot - HRP Anti-Fatty Acid Synthase antibody [EPR7466] (ab196854)](https://www.abcam.com/ps/products/196/ab196854/Images/ab196854-242078-anti-fatty-acid-synthase-antibody-epr7466-hrp-western-blot.jpg)
Western blot - HRP Anti-Fatty Acid Synthase antibody [EPR7466] (ab196854)All lanes : HRP Anti-Fatty Acid Synthase antibody [EPR7466] (ab196854) at 1/5000 dilution
Lane 1 :HeLa whole cell lysate (ab150035)
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 3 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 273 kDa
Observed band size: 273 kDa
Exposure time: 8 minutesThis blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab196854 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
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![HRP Anti-Fatty Acid Synthase antibody [EPR7466] (ab196854)](https://www.abcam.com/ps/products/196/ab196854/Images/ab196854-3-benefits-of-recombinant-antibodies.png)
HRP Anti-Fatty Acid Synthase antibody [EPR7466] (ab196854)
