Antibodies, Proteins, Kits and Reagents for Life Science

331 689 ₸ Product size

100 µl 331 689 ₸

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Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] (ab68191)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP1885Y] to Acetyl Coenzyme A Carboxylase (phospho S79)
Suitable for: Dot blot, WB
Knockout validated
Reacts with: Mouse, Rat, Human

Product name

Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y]
See all Acetyl Coenzyme A Carboxylase primary antibodies
Description

Rabbit monoclonal [EP1885Y] to Acetyl Coenzyme A Carboxylase (phospho S79)
Host species

Rabbit
Specificity

This antibody detects Acetyl Coenzyme A Carboxylase phosphorylated at serine 79.
Tested Applications & Species

Application Species
Dot
Mouse

WB
Mouse

Rat

Human

See all applications and species data
Immunogen

Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Acetyl Coenzyme A Carboxylase (phospho S79). The exact sequence is proprietary.
Positive control
- WB: A431 cell lysate, PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate (treated and untreated), RAW264.7 (mouse abelson murine leukemia virus-induced tumor) whole cell lysate (treated and untreated).
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
Dot	Mouse
WB	Mouse Rat Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EP1885Y
Isotype

IgG
Research areas

Western blot - Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] (ab68191)

All lanes : Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] (ab68191) at 1/5000 dilution

Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : ACACA knockout HAP1 whole cell lysate
Lane 3 : Hek-293 whole cell lysate

Lysates/proteins at 20 µg per lane.

Predicted band size: 277 kDa
Observed band size: 266 kDa
why is the actual band size different from the predicted?

Lanes 1 - 3: Merged signal (red and green). Green - ab68191 observed at 266 kDa. Red - loading control, ab130007, observed at 130 kDa.

ab68191 was shown to specifically react with in wild-type HAP1 cells as signal was lost in ACACA knockout cells. Wild-type and ACACA knockout samples were subjected to SDS-PAGE. Ab68191 and ab130007 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] (ab68191)

All lanes : Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] (ab68191) at 1/1000 dilution

Lane 1 : PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate. Then the membrane was incubated with phosphatase.
Lane 2 : Untreated PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate.

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 277 kDa
Observed band size: 265 kDa why is the actual band size different from the predicted?

Exposure time: 5 seconds

Blocking and diluting buffer: 5% NFDM/TBST
Western blot - Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] (ab68191)

All lanes : Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] (ab68191) at 1/1000 dilution

Lane 1 : RAW264.7 (mouse abelson murine leukemia virus-induced tumor) whole cell lysate. Then the membrane was incubated with phosphatase.
Lane 2 : Untreated RAW264.7 (mouse abelson murine leukemia virus-induced tumor) whole cell lysate.

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 277 kDa
Observed band size: 265 kDa why is the actual band size different from the predicted?

Exposure time: 8 seconds

Blocking and diluting buffer: 5% NFDM/TBST
Western blot - Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] (ab68191)

All lanes : Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] (ab68191) at 1/10000 dilution

Lane 1 : Untreated A431 whole cell lysate
Lane 2 : A431 whole cell lysate - treated with lambda phosphatase

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/2000 dilution

Predicted band size: 277 kDa
Observed band size: 265 kDa why is the actual band size different from the predicted?

Exposure time: 30 seconds

Blocking and dilution buffer: 5% NFDM/TBST.
Dot Blot - Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] (ab68191)

Dot blot analysis of Acetyl Coenzyme A Carboxylase (pS79) phospho peptide (lane 1) and Acetyl Coenzyme A Carboxylase non-phospho peptide (lane 2) labelling Acetyl Coenzyme A Carboxylase (phospho S79) with ab68191 at a dilution of 1/1000. A peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/2500).
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] (ab68191)

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