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Cancer Cell cycle Kinases/phosphatases Cdks

Anti-GSK3 beta (phospho Y216) + GSK3 alpha (phospho Y279) antibody (ab75745)

Price and availability

321 638 ₸

Availability

Order now and get it on Wednesday February 24, 2021

Anti-GSK3 beta (phospho Y216) + GSK3 alpha (phospho Y279) antibody (ab75745)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to GSK3 beta (phospho Y216) + GSK3 alpha (phospho Y279)
  • Suitable for: ICC/IF, WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-GSK3 beta (phospho Y216) + GSK3 alpha (phospho Y279) antibody
  • Description

    Rabbit polyclonal to GSK3 beta (phospho Y216) + GSK3 alpha (phospho Y279)
  • Host species

    Rabbit
  • Specificity

    ab75745 detects endogenous levels of GSK3 beta only when phosphorylated at tyrosine 216 and GSK3 alpha when phosphorylated at tyrosine 276. This antibody can also cross-react with GSK3 alpha subunit due to the consistent antigen-sequence of "GEPNVSYICSRYY".

  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human GSK3 (alpha + beta). Synthetic phosphopeptide derived from human GSK3 beta around the phosphorylation site of tyrosine 216 (V-S-YP-I-C).
    Database link: P49840
    (Peptide available as ab110532)

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 0.87% Sodium chloride, 50% Glycerol, PBS

    Without Mg2+ and Ca2+
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab75745 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Kinases/Phosphatases
    • Cdks
    • Neuroscience
    • Neurology process
    • Notch Pathway
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • Other Kinases
    • Stem Cells
    • Signaling Pathways
    • Wnt
    • Cytoplasmic
    • Stem Cells
    • Signaling Pathways
    • Notch
    • Cytoplasmic
    • Cardiovascular
    • Atherosclerosis
    • Diabetes associated
    • Cardiovascular
    • Heart
    • Apoptosis
    • Cardiovascular
    • Heart
    • Hypertrophy
    • Other
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of carbohydrates
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Integration of energy metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Carbohydrate metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Integration of energy
    • Metabolism
    • Types of disease
    • Diabetes
    • Metabolism
    • Types of disease
    • Cancer
    • Metabolism
    • Types of disease
    • Heart disease

Images

  • Western blot - Anti-GSK3 beta (phospho Y216) + GSK3 alpha (phospho Y279) antibody (ab75745)
    Western blot - Anti-GSK3 beta (phospho Y216) + GSK3 alpha (phospho Y279) antibody (ab75745)
    All lanes : Anti-GSK3 beta (phospho Y216) + GSK3 alpha (phospho Y279) antibody (ab75745) at 1/500 dilution

    Lane 1 : 293 cell extracts, untreated
    Lane 2 : 293 cell extracts, treated with insulin (40µM, 30min)

    Predicted band size: 47 kDa
    Observed band size: 47 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSK3 beta (phospho Y216) + GSK3 alpha (phospho Y279) antibody (ab75745)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSK3 beta (phospho Y216) + GSK3 alpha (phospho Y279) antibody (ab75745)

    ab75745, at a 1/50 dilution, staining GSK3 alpha/beta in paraffin embedded human breast carcinoma tissue by Immunohistochemistry, in the absence (left image) or presence (right image) of the immunizing phospho peptide.

  • Immunocytochemistry/ Immunofluorescence - Anti-GSK3 beta (phospho Y216) + GSK3 alpha (phospho Y279) antibody (ab75745)
    Immunocytochemistry/ Immunofluorescence - Anti-GSK3 beta (phospho Y216) + GSK3 alpha (phospho Y279) antibody (ab75745)

    ab75745 at 1/100 dilution staining GSK3 alpha/beta in human Hela cells by ICC/IF.

  • Western blot - Anti-GSK3 beta (phospho Y216) + GSK3 alpha (phospho Y279) antibody (ab75745)
    Western blot - Anti-GSK3 beta (phospho Y216) + GSK3 alpha (phospho Y279) antibody (ab75745)
    All lanes : Anti-GSK3 beta (phospho Y216) + GSK3 alpha (phospho Y279) antibody (ab75745) at 1/500 dilution

    Lane 1 : 293 cells untreated PMA
    Lane 2 : 293 cells treated PMA

    Predicted band size: 47 kDa

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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