Anti-GSK3 beta (phospho Y216) + GSK3 alpha (phospho Y279) antibody (ab75745)
Key features and details
- Rabbit polyclonal to GSK3 beta (phospho Y216) + GSK3 alpha (phospho Y279)
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-GSK3 beta (phospho Y216) + GSK3 alpha (phospho Y279) antibody -
Description
Rabbit polyclonal to GSK3 beta (phospho Y216) + GSK3 alpha (phospho Y279) -
Host species
Rabbit -
Specificity
ab75745 detects endogenous levels of GSK3 beta only when phosphorylated at tyrosine 216 and GSK3 alpha when phosphorylated at tyrosine 276. This antibody can also cross-react with GSK3 alpha subunit due to the consistent antigen-sequence of "GEPNVSYICSRYY".
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Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.87% Sodium chloride, 50% Glycerol, PBS
Without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab75745 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-GSK3 beta (phospho Y216) + GSK3 alpha (phospho Y279) antibody (ab75745) at 1/500 dilution
Lane 1 : 293 cell extracts, untreated
Lane 2 : 293 cell extracts, treated with insulin (40µM, 30min)
Predicted band size: 47 kDa
Observed band size: 47 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSK3 beta (phospho Y216) + GSK3 alpha (phospho Y279) antibody (ab75745)
ab75745, at a 1/50 dilution, staining GSK3 alpha/beta in paraffin embedded human breast carcinoma tissue by Immunohistochemistry, in the absence (left image) or presence (right image) of the immunizing phospho peptide.
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Immunocytochemistry/ Immunofluorescence - Anti-GSK3 beta (phospho Y216) + GSK3 alpha (phospho Y279) antibody (ab75745)
ab75745 at 1/100 dilution staining GSK3 alpha/beta in human Hela cells by ICC/IF.
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All lanes : Anti-GSK3 beta (phospho Y216) + GSK3 alpha (phospho Y279) antibody (ab75745) at 1/500 dilution
Lane 1 : 293 cells untreated PMA
Lane 2 : 293 cells treated PMA
Predicted band size: 47 kDa