Anti-CDK1 (phospho T161) antibody (ab47329)
Key features and details
- Rabbit polyclonal to CDK1 (phospho T161)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CDK1 (phospho T161) antibody
See all CDK1 primary antibodies -
Description
Rabbit polyclonal to CDK1 (phospho T161) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthesized phosphopeptide derived from human CDC2 around the phosphorylation site of Threonine161 (V-Y-TP-H-E).
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Positive control
- WB: COLO, K362 cell extract. IHC-P: Human breast carcinoma tissue.
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General notes
This antibody detects CDC2 only when phosphorylated at Threonine 161.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The antibody was affinity-purified using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Primary antibody notes
This antibody detects CDC2 only when phosphorylated at Threonine 161. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-CDK1 (phospho T161) antibody (ab47329)All lanes : Anti-CDK1 (phospho T161) antibody (ab47329) at 1/500 dilution
Lane 1 : COLO cell extract preincubated with immunogenic phospho-peptide. with phosphopeptide at 1 µg/ml
Lane 2 : COLO cell extract, untreated.
Lane 3 : K362 cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 34 kDa
Observed band size: 34 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK1 (phospho T161) antibody (ab47329)ab47329 staining human breast carcinoma tissue by IHC-P (left hand panel). The right hand panel shows staining in the presence of synthesized phospho-peptide using Alkaline phosphatase Goat anti rabbit IgG (H+L) secondary antibody.
