Anti-SLC4A7/NBCn1 antibody (ab82335)
Key features and details
- Rabbit polyclonal to SLC4A7/NBCn1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SLC4A7/NBCn1 antibody
See all SLC4A7/NBCn1 primary antibodies -
Description
Rabbit polyclonal to SLC4A7/NBCn1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human SLC4A7/NBCn1 aa 1150 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab96634) -
Positive control
- This antibody gave a positive signal in the following whole cell lysates: Caco2 and HEK293.
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General notes
This product was previously labelled as SLC4A7
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab82335 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species IHC-P HumanWB HumanApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 136 kDa (predicted molecular weight: 136 kDa).IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 136 kDa (predicted molecular weight: 136 kDa).IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.Target
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Function
Electroneutral sodium- and bicarbonate-dependent cotransporter with a Na(+):HCO3(-) 1:1 stoichiometry. Regulates intracellular pH and may play a role in bicarbonate salvage in secretory epithelia. May also have an associated sodium channel activity. -
Tissue specificity
Highly expressed in testis and spleen. Also expressed in retina, colon, small intestine, ovary, thymus, prostate, muscle, heart and kidney. Isoform 1 is expressed in skeletal muscle and heart muscle. -
Sequence similarities
Belongs to the anion exchanger (TC 2.A.31) family. -
Domain
The PDZ-binding motif mediates interaction with the CFTR, SLC9A3R1/EBP50 complex and probably with USH1C. -
Cellular localization
Basolateral cell membrane. Apical cell membrane. Cell projection, stereocilium. Also described at the apical cell membrane. Localizes to the stereocilia of cochlear outer hair cells and to the lateral membrane of cochlear inner hair cells (By similarity). - Information by UniProt
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Database links
- Entrez Gene: 9497 Human
- Omim: 603353 Human
- SwissProt: Q9Y6M7 Human
- Unigene: 250072 Human
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Alternative names
- Bicarbonate transporter antibody
- BT antibody
- Electroneutral Na/HCO(3) cotransporter antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC4A7/NBCn1 antibody (ab82335)
IHC image of SLC4A7/NBCn1 staining in human normal kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab82335, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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Western blot - Anti-SLC4A7/NBCn1 antibody (ab82335)All lanes : Anti-SLC4A7/NBCn1 antibody (ab82335) at 1 µg/ml
Lane 1 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 136 kDa
Observed band size: 136 kDa
Additional bands at: 19 kDa, 60 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
Datasheets and documents
References (4)
ab82335 has been referenced in 4 publications.
- Hwang S et al. Drug Repurposing as an Antitumor Agent: Disulfiram-Mediated Carbonic Anhydrase 12 and Anion Exchanger 2 Modulation to Inhibit Cancer Cell Migration. Molecules 24:N/A (2019). PubMed: 31546841
- Xie ZD et al. The Balance of [Formula: see text] Secretion vs. Reabsorption in the Endometrial Epithelium Regulates Uterine Fluid pH. Front Physiol 9:12 (2018). WB, IHC-Fr . PubMed: 29422866
- Sedlyarov V et al. The Bicarbonate Transporter SLC4A7 Plays a Key Role in Macrophage Phagosome Acidification. Cell Host Microbe 23:766-774.e5 (2018). PubMed: 29779931
- Namkoong E et al. Role of Sodium Bicarbonate Cotransporters in Intracellular pH Regulation and Their Regulatory Mechanisms in Human Submandibular Glands. PLoS One 10:e0138368 (2015). Human . PubMed: 26375462
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC4A7/NBCn1 antibody (ab82335)
IHC image of SLC4A7/NBCn1 staining in human normal kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab82335, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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Western blot - Anti-SLC4A7/NBCn1 antibody (ab82335)All lanes : Anti-SLC4A7/NBCn1 antibody (ab82335) at 1 µg/ml
Lane 1 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 136 kDa
Observed band size: 136 kDa
Additional bands at: 19 kDa, 60 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
