Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Ghrelin antibody [EPR20502] - BSA and Azide free (ab271988)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20502] to Ghrelin - BSA and Azide free
  • Suitable for: IHC-Fr, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Ghrelin antibody [EPR20502] - BSA and Azide free
    See all Ghrelin primary antibodies

  • Description

    Rabbit monoclonal [EPR20502] to Ghrelin - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-Fr, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human, mouse and rat stomach tissues. IHC-Fr: Mouse stomach tissue.

  • General notes

    ab271988 is the carrier-free version of ab209790. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ghrelin antibody [EPR20502] - BSA and Azide free (ab271988)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ghrelin antibody [EPR20502] - BSA and Azide free (ab271988)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue labeling Ghrelin with ab209790 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on endocrine cells of human stomach is observed [PMID: 18474938]. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209790).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ghrelin antibody [EPR20502] - BSA and Azide free (ab271988)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ghrelin antibody [EPR20502] - BSA and Azide free (ab271988)

    Immunohistochemical analysis of paraffin-embedded human liver tissue labeling Ghrelin with ab209790 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Negative control: no staining on human liver [PMID: 10604470].

    Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209790).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ghrelin antibody [EPR20502] - BSA and Azide free (ab271988)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ghrelin antibody [EPR20502] - BSA and Azide free (ab271988)

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue labeling Ghrelin with ab209790 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on endocrine cells of mouse stomach is observed [PMID: 18474938]. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209790).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ghrelin antibody [EPR20502] - BSA and Azide free (ab271988)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ghrelin antibody [EPR20502] - BSA and Azide free (ab271988)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling Ghrelin with ab209790 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Negative control: no staining on mouse liver [PMID: 10604470].

    Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209790).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ghrelin antibody [EPR20502] - BSA and Azide free (ab271988)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ghrelin antibody [EPR20502] - BSA and Azide free (ab271988)

    Immunohistochemical analysis of paraffin-embedded rat stomach tissue labeling Ghrelin with ab209790 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on endocrine cells of rat stomach is observed [PMID: 18474938]. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209790).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ghrelin antibody [EPR20502] - BSA and Azide free (ab271988)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ghrelin antibody [EPR20502] - BSA and Azide free (ab271988)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling Ghrelin with ab209790 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Negative control: no staining on rat liver [PMID: 10604470].

    Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209790).
  • Immunohistochemistry (Frozen sections) - Anti-Ghrelin antibody [EPR20502] - BSA and Azide free (ab271988)
    Immunohistochemistry (Frozen sections) - Anti-Ghrelin antibody [EPR20502] - BSA and Azide free (ab271988)

    Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse stomach tissue labeling Ghrelin with ab209790 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Cytoplasmic staining on endocrine cells of mouse stomach is observed [PMID: 18474938]. The nuclear counterstain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

    This antibody worked well on mouse IHC-Frozen sections. Staining on rat sections was weak and human samples were not tested.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209790).
  • Anti-Ghrelin antibody [EPR20502] - BSA and Azide free (ab271988)
    Anti-Ghrelin antibody [EPR20502] - BSA and Azide free (ab271988)

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