Antibodies, Proteins, Kits and Reagents for Life Science

267 361 ₸ Product size

100 µl 267 361 ₸ 10 µl 66 337 ₸

Order now and get it on Tuesday March 16, 2021

Anti-PLBD2 antibody [IGX4128H] (ab213295)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Human monoclonal [IGX4128H] to PLBD2
Suitable for: WB, ELISA
Reacts with: Mouse, Human

Product name

Anti-PLBD2 antibody [IGX4128H]
See all PLBD2 primary antibodies
Description

Human monoclonal [IGX4128H] to PLBD2
Host species

Human
Tested Applications & Species

Application Species
ELISA
Human

WB
Mouse

See all applications and species data
Immunogen

Recombinant fragment. This information is considered to be commercially sensitive.
Positive control
- WB: Mouse Cerebellum tissue lysate
General notes

This product was made using synthetic libraries and phage display technology.

This antibody is a recombinant antibody.
Human monoclonal antibody.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Application	Species
ELISA	Human
WB	Mouse

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Constituents: 0.484% Tris buffer, 0.45% Glycine, 40% Glycerol, 1.044% Sodium chloride
Concentration information loading...
Purity

Immunogen affinity purified
Clonality

Monoclonal
Clone number

IGX4128H
Isotype

IgG1
Research areas

ELISA - Anti-PLBD2 antibody [IGX4128H] (ab213295)

Different concentrations of immobilised recombinant fragment of human PLBD2 was recognised in an indirect ELISA by ab213295 (1 µg x mL^-1). The antibody was bound by peroxidase conjugated recombinant protein A at 0.2 µg x mL^-1. OD (background signal subtracted) shown on the y-axis.
Western blot - Anti-PLBD2 antibody [IGX4128H] (ab213295)

Anti-PLBD2 antibody [IGX4128H] (ab213295) at 1 µg/ml + Mouse Cerebellum Tissue Lysate at 10 µg

Secondary
HRP conjugated Goat Anti-Human IgG (H+L) at 1/10000 dilution

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 65 kDa
Additional bands at: 42 kDa (possible cleavage fragment)

Exposure time: 20 minutes

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab213295 overnight at 4°C. Antibody binding was detected using an anti-human antibody conjugated to HRP, and visualised using ECL development solution ab133406.
Anti-PLBD2 antibody [IGX4128H] (ab213295)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com