Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PLBD2 antibody [IGX4128H] (ab213295)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Human monoclonal [IGX4128H] to PLBD2
  • Suitable for: WB, ELISA
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-PLBD2 antibody [IGX4128H]
    See all PLBD2 primary antibodies

  • Description

    Human monoclonal [IGX4128H] to PLBD2

  • Host species

    Human

  • Tested Applications & Species

    Application Species
    ELISA
    Human
    WB
    Mouse
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is considered to be commercially sensitive.

  • Positive control

    • WB: Mouse Cerebellum tissue lysate

  • General notes

    This product was made using synthetic libraries and phage display technology.

    This antibody is a recombinant antibody. 
    Human monoclonal antibody.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Constituents: 0.484% Tris buffer, 0.45% Glycine, 40% Glycerol, 1.044% Sodium chloride
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Monoclonal

  • Clone number

    IGX4128H

  • Isotype

    IgG1

  • Research areas

Images

  • ELISA - Anti-PLBD2 antibody [IGX4128H] (ab213295)
    ELISA - Anti-PLBD2 antibody [IGX4128H] (ab213295)
     Different concentrations of immobilised recombinant fragment of human PLBD2 was recognised in an indirect ELISA by ab213295 (1 µg x mL-1). The antibody was bound by peroxidase conjugated recombinant protein A at 0.2 µg x mL-1. OD (background signal subtracted) shown on the y-axis.
  • Western blot - Anti-PLBD2 antibody [IGX4128H] (ab213295)
    Western blot - Anti-PLBD2 antibody [IGX4128H] (ab213295)
    Anti-PLBD2 antibody [IGX4128H] (ab213295) at 1 µg/ml + Mouse Cerebellum Tissue Lysate at 10 µg

    Secondary
    HRP conjugated Goat Anti-Human IgG (H+L) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 65 kDa
    Additional bands at: 42 kDa (possible cleavage fragment)


    Exposure time: 20 minutes


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab213295 overnight at 4°C. Antibody binding was detected using an anti-human antibody conjugated to HRP, and visualised using ECL development solution ab133406.

  • Anti-PLBD2 antibody [IGX4128H] (ab213295)
    Anti-PLBD2 antibody [IGX4128H] (ab213295)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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