Anti-PLBD2 antibody [EPR21742] - BSA and Azide free (ab231825)
![Anti-PLBD2 antibody [EPR21742] - BSA and Azide free (ab231825)](https://www.abcam.com/ps/products/231/ab231825/Images/ab231825-302439-216866.jpg "Anti-PLBD2 antibody [EPR21742] - BSA and Azide free (ab231825)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR21742] to PLBD2 - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-PLBD2 antibody [EPR21742] - BSA and Azide free
See all PLBD2 primary antibodies -
Description
Rabbit monoclonal [EPR21742] to PLBD2 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: U937, MCF7, MEF and NIH/3T3 whole cell lysates; human fetal spleen, fetal kidney and fetal brain tissue lysates.
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General notes
Ab231825 is the carrier-free version of ab216866. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab231825 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR21742 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PLBD2 antibody [EPR21742] - BSA and Azide free (ab231825)All lanes : Anti-PLBD2 antibody [EPR21742] (ab216866) at 1/1000 dilution
Lane 1 : U937 (human histiocytic lymphoma cell line) whole cell lysate at 20 µg
Lane 2 : Human fetal spleen tissue lysate at 20 µg
Lane 3 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 20 µg
Lane 4 : MEF (mouse embryonic fibroblast cell line) whole cell lysate at 20 µg
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 20 µg
Lane 6 : Human fetalkidney tissue lysate at 20 µg
Lane 7 : Human fetal brain tissue lysate at 10 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 65 kDa
Observed band size: 76 kDa why is the actual band size different from the predicted?
Additional bands at: 32/28 kDa (possible cleavage fragment)Blocking and dilution buffer: 5% NFDM/TBST.
Exposure times.
Lanes 1 & 2: 59 seconds
Lane 3: 15 seconds.
Lanes 4 & 5: 3 seconds.
Lanes 6 & 7: 3 minutes.
Full-length PLBD2 (76 kDa) can be cleaved into N-terminal and C-terminal fragments according to the literature (PMID:17007843, PMID: 17105447).
This antibody recognizes both full-length and the N-terminal fragment of PLBD2.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab216866).
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![Anti-PLBD2 antibody [EPR21742] - BSA and Azide free (ab231825)](https://www.abcam.com/ps/products/231/ab231825/Images/ab231825-2-benefits-of-recombinant-antibodies.png)
Anti-PLBD2 antibody [EPR21742] - BSA and Azide free (ab231825)
