Isocitrate Dehydrogenase Assay Kit (Colorimetric) (ab102528)
Key features and details
- Assay type: Enzyme activity
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 45 min
- Sample type: Cell culture supernatant, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
- Sensitivity: 0.01 mU/well
Overview
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Product name
Isocitrate Dehydrogenase Assay Kit (Colorimetric)
See all Isocitrate Dehydrogenase kits -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts -
Assay type
Enzyme activity -
Sensitivity
> 0.01 mU/well -
Assay time
0h 45m -
Product overview
Abcam's Isocitrate Dehydrogenase Assay Kit (Colorimetric) provides a convenient tool for sensitive detection of NAD(+) / NADP(+)-dependent or both IDHs in a variety of samples. The IDHs utilize isocitrate as a specific substrate leading to a proportional color development and can be easily quantified colorimetrically (λ = 450 nm) with detection sensitivity as low as 0.01 mU.
Visit our FAQs page for tips and troubleshooting.Isocitrate dehydrogenase assay protocol summary:
- add samples and standards to wells
- incubate for 3 min and analyze with microplate reader
- incubate for 30 min - 2 hr and analyze again -
Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 100 tests Developer (lyophilized) Purple 1 vial IDH Assay Buffer WM 1 x 25ml IDH Positive Control (NADP+) Green 1 x 20µl IDH Substrate (lyophilized) Red 1 vial NAD+ (lyophilized) Blue 1 vial NADH Standard ( lyophilized) Yellow 1 vial NADP+ (lyophilized) Brown 1 vial -
Research areas
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Relevance
Isocitrate dehydrogenase (IDH; EC 1.1.1.41, NAD+) is an enzyme that participates in the citric acid cycle. These IDH3 isoforms catalyze the oxidative decarboxylation of isocitrate, producing alpha-ketoglutarate and CO2 while converting NAD+ to NADH. This is a two-step process, which involves oxidation of isocitrate to oxalosuccinate, followed by the decarboxylation of the beta-carboxyl group to form the ketone, alpha-ketoglutarate. Other isoforms (EC 1.1.1.42, NADP+) catalyze the same reaction, but unrelated to the citric acid cycle. It is carried out in the mitochondrion (IDH2) as well as in the cytosol and peroxisome (IDH1) and use NADP+ as a cofactor instead of NAD+. -
Cellular localization
Cytoplasm. Peroxisome -
Alternative names
- Cytosolic NADP isocitrate dehydrogenase
- EC=1.1.1.42
- IDH
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Images
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Functional Studies - Isocitrate Dehydrogenase Detection Kit (ab102528)Isocitrate Dehydrogenase detection in Bovine Liver Extraction Sample using ab102528
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Functional Studies - Isocitrate Dehydrogenase Detection Kit (ab102528)Example of NADH and NADPH Standard Curve obtained using ab102528