Antibodies, Proteins, Kits and Reagents for Life Science

264 681 ₸ Product size

100 µl 264 681 ₸

Order now and get it on Wednesday March 17, 2021

Anti-Map17 antibody [EPR16827] (ab199540)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR16827] to Map17
Suitable for: WB, IP
Reacts with: Human

Product name

Anti-Map17 antibody [EPR16827]
See all Map17 primary antibodies
Description

Rabbit monoclonal [EPR16827] to Map17
Host species

Rabbit
Tested Applications & Species

Application Species
IP
Human

WB
Human

See all applications and species data
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- Human fetal kidney lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Application	Species
IP	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR16827
Isotype

IgG
Research areas

Western blot - Anti-Map17 antibody [EPR16827] (ab199540)

All lanes : Anti-Map17 antibody [EPR16827] (ab199540) at 1/10000 dilution

Lane 1 : Human fetal kidney lysate
Lane 2 : Human fetal brain lysate
Lane 3 : Human fetal heart lysate
Lane 4 : Human fetal spleen lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 12 kDa
Observed band size: 17 kDa
why is the actual band size different from the predicted?

Exposure time: 5 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.
The observed MW is consistent with what has been described in the literature PMID: 10951271.
Human fetal brain, Human fetal heart, Human fetal spleen represent negative controls for Map17.
Immunoprecipitation - Anti-Map17 antibody [EPR16827] (ab199540)

Map17 was immunoprecipitated from 1mg of Human fetal kidney whole cell lysate with ab199540 at 1/120 dilution. Western blot was performed from the immunoprecipitate using ab199540 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
Lane 1: Human fetal kidney whole cell lysate 10ug (Input). Lane 2: ab199540 IP in Human fetal kidney whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab199540 in Human fetal kidney whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds.
Immunoprecipitation - Anti-Map17 antibody [EPR16827] (ab199540)

Map17 was immunoprecipitated from 1mg of Human fetal kidney whole cell lysate with ab199540 at 1/300 dilution. Western blot was performed from the immunoprecipitate using anti-Map17 (ab156014) at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
Lane 1: Human fetal kidney whole cell lysate 10ug (Input). Lane 2: ab199540 IP in Human fetal kidney whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab199540 in Human fetal kidney whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.
Anti-Map17 antibody [EPR16827] (ab199540)

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