Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Map17 antibody [EPR16827] (ab199540)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16827] to Map17
  • Suitable for: WB, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-Map17 antibody [EPR16827]
    See all Map17 primary antibodies

  • Description

    Rabbit monoclonal [EPR16827] to Map17

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human fetal kidney lysate.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR16827

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Map17 antibody [EPR16827] (ab199540)
    Western blot - Anti-Map17 antibody [EPR16827] (ab199540)
    All lanes : Anti-Map17 antibody [EPR16827] (ab199540) at 1/10000 dilution

    Lane 1 : Human fetal kidney lysate
    Lane 2 : Human fetal brain lysate
    Lane 3 : Human fetal heart lysate
    Lane 4 : Human fetal spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 12 kDa
    Observed band size: 17 kDa
    why is the actual band size different from the predicted?


    Exposure time: 5 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The observed MW is consistent with what has been described in the literature PMID: 10951271.

    Human fetal brain, Human fetal heart, Human fetal spleen represent negative controls for Map17.

  • Immunoprecipitation - Anti-Map17 antibody [EPR16827] (ab199540)
    Immunoprecipitation - Anti-Map17 antibody [EPR16827] (ab199540)

    Map17 was immunoprecipitated from 1mg of Human fetal kidney whole cell lysate with ab199540 at 1/120 dilution. Western blot was performed from the immunoprecipitate using ab199540 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

    Lane 1: Human fetal kidney whole cell lysate 10ug (Input). Lane 2: ab199540 IP in Human fetal kidney whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab199540 in Human fetal kidney whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds.

  • Immunoprecipitation - Anti-Map17 antibody [EPR16827] (ab199540)
    Immunoprecipitation - Anti-Map17 antibody [EPR16827] (ab199540)

    Map17 was immunoprecipitated from 1mg of Human fetal kidney whole cell lysate with ab199540 at 1/300 dilution. Western blot was performed from the immunoprecipitate using anti-Map17 (ab156014) at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

    Lane 1: Human fetal kidney whole cell lysate 10ug (Input). Lane 2: ab199540 IP in Human fetal kidney whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab199540 in Human fetal kidney whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds.

  • Anti-Map17 antibody [EPR16827] (ab199540)
    Anti-Map17 antibody [EPR16827] (ab199540)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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