Anti-FNDC3A antibody (ab254706)
Key features and details
- Rabbit polyclonal to FNDC3A
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
-
Product name
Anti-FNDC3A antibody
See all FNDC3A primary antibodies -
Description
Rabbit polyclonal to FNDC3A -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Orangutan -
Immunogen
Recombinant fragment corresponding to Human FNDC3A aa 100-300.
Database link: Q9Y2H6 -
Positive control
- IHC-P: Human placenta, rectum, testis and liver tissue. ICC/IF: U-251 MG cells. WB: RT4 and U-251 MG cell lysate. Human liver and tonsil tissue lysate.
-
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
PFA fixed, Triton X-100 permeabilized U-251 MG (Human brain glioma cell line) cells labeling FNDC3A using ab254706 at 4µg/ml (green) in ICC/IF.
-
Paraffin-embedded human placenta tissue stained for FNDC3A using ab254706 at 1/500 dilution in immunohistochemical analysis.
-
Lanes 2-6 : Anti-FNDC3A antibody (ab254706) at 0.4 µg/ml
Lane 1 : MW ladder
Lane 2 : RT4 cell lysate
Lane 3 : U-251 MG cell lysate
Lane 4 : Human plasma (IgG/HSA depleted)
Lane 5 : Human liver tissue lysate
Lane 6 : Human tonsil tissue lysate
-
All lanes : Anti-FNDC3A antibody (ab254706) at 0.4 µg/ml
Lane 1 : NIH/3T3 cell lysate
Lane 2 : NBT-II cell lysate
-
Paraffin-embedded human rectum tissue stained for FNDC3A using ab254706 at 1/500 dilution in immunohistochemical analysis.
-
Paraffin-embedded human testis tissue stained for FNDC3A using ab254706 at 1/500 dilution in immunohistochemical analysis.
-
Paraffin-embedded human liver tissue stained for FNDC3A using ab254706 at 1/500 dilution in immunohistochemical analysis.