Human GDF11 ELISA Kit (ab267609)
Key features and details
- Sensitivity: 0.16 ng/ml
- Range: 0.164 ng/ml - 40 ng/ml
- Sample type: Cell culture media, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human GDF11 ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Serum, Plasma, Cell culture media -
Assay type
Sandwich (quantitative) -
Sensitivity
0.16 ng/ml -
Range
0.164 ng/ml - 40 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 115.7 111% - 122% Plasma 98.47 80% - 117% Cell culture media 90.79 83% - 97% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human GDF11 ELISA Kit is designed for the quantitative determination of GDF11 in cell culture supernatants, plasma and serum samples.
ΔNote: Human GDF11 concentration is low in normal serum/plasma, and may not be detectable in this assay.
This assay employs an antibody specific for human GDF11 coated on a 96-well plate. Standards and samples are pipetted into the wells and GDF11 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human GDF11 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of GDF11 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml 700X HRP-Streptavidin Concentrate 1 x 200µl Anti-Human GDF11 coated Microplate (12 x 8 wells) 1 unit Assay Diluent C 1 x 30ml Biotinylated Anti-Human GDF11 Detection Antibody 2 vials Human GDF11 Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
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Function
Secreted signal that acts globally to specify positional identity along the anterior/posterior axis during development. Play critical roles in patterning both mesodermal and neural tissues and in establishing the skeletal pattern. -
Sequence similarities
Belongs to the TGF-beta family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- BMP 11
- BMP-11
- BMP11
see all -
Database links
- Entrez Gene: 10220 Human
- Omim: 603936 Human
- SwissProt: O95390 Human
- Unigene: 600883 Human
Images
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Example standard curve
This standard curve is for demonstration only. A standard curve must be run with each assay.