Antibodies, Proteins, Kits and Reagents for Life Science

257 980 ₸ Product size

100 µl 257 980 ₸

Order now and get it on Wednesday March 10, 2021

Anti-GDF7 antibody [EPR16000] (ab189928)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR16000] to GDF7
Suitable for: WB, IHC-P, ICC/IF
Reacts with: Mouse, Rat, Human

Product name

Anti-GDF7 antibody [EPR16000]
See all GDF7 primary antibodies
Description

Rabbit monoclonal [EPR16000] to GDF7
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Human
WB	Rat Human

See all applications and species data

Immunogen

This information is proprietary to Abcam and/or its suppliers.
Positive control
- Human fetal brain, C6 and Neuro-2a cell lysates; Human adenocarcinoma of colon and rat brain tissues, U87-MG cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR16000
Isotype

IgG
Research areas

Immunocytochemistry/ Immunofluorescence - Anti-GDF7 antibody [EPR16000] (ab189928)

Immunocytochemical analysis of 4% paraformaldehyde fixed U87-MG cells labeling GDF7 with ab189928 at 1/50 dilution, and Goat anti rabbit IgG(Alexa Fluor® 555) at 1/200 as a secondary antibody. Counterstained with DAPI.
Western blot - Anti-GDF7 antibody [EPR16000] (ab189928)

Anti-Spa-1 antibody [EPR14134] (ab189929) at 1/10000 dilution + Human fetal brain at 20 µg

Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 47 kDa
Additional bands at: 52 kDa. We are unsure as to the identity of these extra bands.
Western blot - Anti-GDF7 antibody [EPR16000] (ab189928)

All lanes : Anti-GDF7 antibody [EPR16000] (ab189928) at 1/1000 dilution

Lane 1 : C6 cell lysate
Lane 2 : Neuro-2a cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 47 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GDF7 antibody [EPR16000] (ab189928)

Immunohistochemical analysis of formalin fixed, paraffin embedded Human adenocarcinoma of colon tissue labeling GDF7 with ab189928 at 1/2000 dilution, and prediluted HRP Polymer for Rabbit IgG as a secondary antibody. Counterstained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GDF7 antibody [EPR16000] (ab189928)

Immunohistochemical analysis of formalin fixed, paraffin embedded Human adenocarcinoma of colon labeling GDF7 with ab189928 at 1/2000 dilution, and prediluted HRP Polymer for Rabbit IgG as a secondary antibody. Counterstained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Anti-GDF7 antibody [EPR16000] (ab189928)

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