Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-GDF7 antibody [EPR16000] (ab189928)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16000] to GDF7
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-GDF7 antibody [EPR16000]
    See all GDF7 primary antibodies

  • Description

    Rabbit monoclonal [EPR16000] to GDF7

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Rat
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human fetal brain, C6 and Neuro-2a cell lysates; Human adenocarcinoma of colon and rat brain tissues, U87-MG cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-GDF7 antibody [EPR16000] (ab189928)
    Immunocytochemistry/ Immunofluorescence - Anti-GDF7 antibody [EPR16000] (ab189928)

    Immunocytochemical analysis of 4% paraformaldehyde fixed U87-MG cells labeling GDF7 with ab189928 at 1/50 dilution, and Goat anti rabbit IgG(Alexa Fluor® 555) at 1/200 as a secondary antibody. Counterstained with DAPI.

  • Western blot - Anti-GDF7 antibody [EPR16000] (ab189928)
    Western blot - Anti-GDF7 antibody [EPR16000] (ab189928)
    Anti-Spa-1 antibody [EPR14134] (ab189929) at 1/10000 dilution + Human fetal brain at 20 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 47 kDa
    Additional bands at: 52 kDa. We are unsure as to the identity of these extra bands.

  • Western blot - Anti-GDF7 antibody [EPR16000] (ab189928)
    Western blot - Anti-GDF7 antibody [EPR16000] (ab189928)
    All lanes : Anti-GDF7 antibody [EPR16000] (ab189928) at 1/1000 dilution

    Lane 1 : C6 cell lysate
    Lane 2 : Neuro-2a cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 47 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GDF7 antibody [EPR16000] (ab189928)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GDF7 antibody [EPR16000] (ab189928)

    Immunohistochemical analysis of formalin fixed, paraffin embedded Human adenocarcinoma of colon tissue labeling GDF7 with ab189928 at 1/2000 dilution, and prediluted HRP Polymer for Rabbit IgG as a secondary antibody. Counterstained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GDF7 antibody [EPR16000] (ab189928)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GDF7 antibody [EPR16000] (ab189928)

    Immunohistochemical analysis of formalin fixed, paraffin embedded Human adenocarcinoma of colon labeling GDF7 with ab189928 at 1/2000 dilution, and prediluted HRP Polymer for Rabbit IgG as a secondary antibody. Counterstained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-GDF7 antibody [EPR16000] (ab189928)
    Anti-GDF7 antibody [EPR16000] (ab189928)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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